Raising the Bar-coding
Where our samples were taken to be sequenced.
What is UTHSC?
Liquid used to coat adhesion plates for platelet binding.
What is Platelet Rich Plasma?
Type of cell used for pGLO transformation.
What is E. coli?
Temperature at which DNA denatures during PCR.
What is 95oC?
Done to keep a tube sterile before pipetting out or into it.
What is flame the top of the tube?
Gene we amplified for barcoding?
What is 16S rRNA?
A non-platelet negative control for the adhesion assay.
What is the Red Blood cell?
An ion solution that makes bacterial cells more permeable to plasmid.
What is calcium?
A characteristic of the interaction between DNA and the resin of spin columns.
What is hydrophobic?
Used as a loading dye for our DNA.
What is EZ vision?
The purpose of Barcoding.
What is identification of unknown bacteria?
Agonist used for our platelet assays.
What is ADP?
The protein allowing transformed cells can grow on ampicillin.
What is beta lactamase?
Identity of what is shown in the photo.
What is 16SrRNA gene fragment?
Describes the buffer that emporarily denatures GFP so that it will bind to the GFP column.
What is a high salt buffer?
Technique performed with our bacterial DNA so it could be seen on our gels.
What is PCR?
Potential inhibitor of platelet adhesion.
What is Aspirin?
The gene that is transcribed when the pGLO arabinose operon is activated.
What is GFP?
Protein that synthesizes DNA during PCR.
What is Taq Polymerase
Dye used for adhesion assay that is pH sensitive.
What is bromophenol blue?
Used to break open bacterial cells for DNA isolation.
What is Proteinase K?
Platelet proteins that remain on the plate after the platelet membrane is solubilized.
What are GP IIb-IIIa or cytoskeletal proteins?
A regulatory gene encoding a protein that can turn off or on the arabinose operon.
What is Ara C?
The reason why we isolated and sequenced a fragment of the 16S rRNA gene from our bacteria.
What is sequence variability among bacteria?
Substance used to solubilize platelet membranes.
What is Trichloroacetic acid?