RB1 & Retinoblastoma
Tumor Biology
Genetics / Inheritance / Presentation
Imaging & Diagnosis
Treatment & Surveillance
100

What is the main function of pRB in the cell cycle? 

pRB inhibits cell cycle progression by inactivating E2F and inhibiting downstream transcription (G1 to S). 

100

What is the "two-hit hypothesis"

Both alleles of a tumor suppressor gene must be inactivated for cancer to develop.

100

What type of inheritance pattern does heritable retinoblastoma follow?



Autosomal dominant

100

What imaging modality is preferred for diagnosing intraocular retinoblastoma?

MRI

100

What surgical treatment is used when vision cannot be preserved in retinoblastoma?

Enucleation

200

What clinical sign is most commonly associated with retinoblastoma in children?

Leukocoria (white pupillary reflex)

200

What's the difference between a tumor suppressor gene and a proto-oncogene?

Tumor suppressors prevent cell growth (loss leads to cancer), while proto-oncogenes promote growth and become oncogenes when mutated

200

What is the risk that a child of a retinoblastoma survivor with a germline RB1 mutation will inherit the mutation?

50%

200

What is the hallmark finding of retinoblastoma on MRI?

An intraocular mass with calcifications and variable contrast enhancement.

200

How is chemotherapy typically delivered (targeted fashion)?

The catheter is threaded through the femoral artery into the ophthalmic artery. 

300

Which kinase complex(s) are responsible for pRB phosphorylation, and at what timepoints? 

Cyclin D–CDK4/6: Early-mid G1 

Cyclin E-CDK2: Late G1 (Near Restriction Point)

300

What is bilateral retinoblastoma suggestive of?



Heritable form of the disease (germline to second mutation)

300

How could a child develop heritable retinoblastoma without a family history?

A de novo germline mutation in the RB1 gene during gametogenesis

300

What is the difference between T1 and T2 imaging, and when/what is the role of contrast?

T1 helps with anatomy, T2 highlights fluid and edema; 

Contrast allows us to see "active" parts of the tumor and areas of spread. 

300

What drugs make up the SOC for Rb, and what are their mechanisms?

VEC-

Vincristine: Inhibits microtubule formation

Etoposide: Inhibits topoisomerase II

Carboplatin: Forms DNA crosslinks 

400

How can p53 compensate for pRB loss?

Increased E2F leads to decreased MDM2, which leads to increased p53. 

400

Why can dominant-negative mutations lead to a more severe phenotype than null alleles?


Dominant-negative mutants may still bind/sequester other interacting proteins.

400

What are the three most common symptoms of Retinoblastoma?

Leukocoria, Strabismus, Eye pain/redness. 

400

What is trilateral retinoblastoma?

Bilateral intraocular tumors plus a midline intracranial tumor

400

What is another cancer associated with Rb mutations that typically affects adolescents and elderly patients?  

Osteosarcoma


500

How does loss of RB1 function contribute to genomic instability, BESIDES unchecked cell growth?

pRB ALSO regulates chromatin remodeling of E2F-associated genes. 

500

Why is the tumor spectrum of Rb1 mutations lower than you'd expect?

High proliferative nature of retinal tissue in early infancy. Compensatory pathways of p107 and p130 in other tissues. 

500

What portion of the chromosome is deleted in some cases of Rb? 

Chromosome 13q14

500

Why is CT scan avoided in retinoblastoma diagnosis, particularly in children?

Risk of radiation-induced secondary malignancies

500

What considerations are made when deciding on a treatment regimen? (Melphalan vs VEC)

Stage, uni- or bi- lateral, tumor location.