Paper 1
Neomycin sulfate is commonly found in this topical antibiotic
Neosporin
What is polydactyly?
a limb malformation characterized by an abnormal number of digits
In the first paper, ICR stands for...
Imprinted Control Region
"Our goal is to figure out a therapy for prion diseases"
Stanley Pruisner
The two types of histone modification are...
Histone methylation and acetylation
What is CRISPR/Cas9?
he method used in the paper to induce deletions, duplications, and inversions in mouse embryonic stem cells
What is sgRNA (or gRNA)?
molecule responsible for guiding Cas9 to the correct location
The structure of our CTCF transcription factor is...
Zinc Finger protein
The popular laboratory technique, EMSA, stands for...
Electrophoretic Mobility Shift Assay
What is a boundary?
a region of the genome that encloses TADs and serves as a binding site for CTCF
Daily Double: What is the name of the known insulator used as a reference or positive control in many of the experiments?
Beta-globin
Self-awareness is preached by this COM program director
Dr. Sayeski
Final Jeopardy: Borussia Dortmund is this COM professors favorite football team.
Dr. Bungert
Beckwith-Wiedemann Syndrome is characterized by tongue and facial overgrowth resulting from pathologic expansion of tissues. This syndrome is also known as...
Fetal Overgrowth Syndrome
What is 4C?
“one vs. all” method of studying chromatin interactions in the genome
What are ectopic interactions?
abnormal interactions between TADs
Discovered a class of enzymes in which enzymes catalyze the reaction to convert chemical bond energy into mechanical work (w= F * d).
Daniel Purich
In the final model proposed by the paper, they suggest that methylated ICR means CTCF (can/can’t) bind to the ICR. The enhancer can therefore (activate/deactivate) Igf2. This is found on the ----- strand.
can’t, activate. This is found on the paternal strand.
What is EPHA4?
deletion of this gene leads to phenotypic expression of brachydactyly in humans
What three organisms did the researchers study when looking at the conserved/consensus sequence?
Human, Mouse, and Rat
Examining the relative number of bacteria colonies formed, when the DMD fragment was place in between our gene and enhancer we observed...
gene silencing/insulation