Mystery
What is an example of a commonly used convex lens?
glasses
*microscope too
Wave travels through the object.
transmission
In a gram stain, which one is pink and which one is purple?
pink = gram-negative
purple = gram-positive
What is the main thing that a fluorescence microscope used for?
tell the difference between live and dead cells
shorter wavelength means...
HIGHER resolution
Define reflection.
(how does the wave move)
wave bounces off material
*The color that we see is actually due to all the other colors being absorbed while the present color is reflected to your eye.
Does a TEM stain used live or dead specimens?
DEAD
(due to cutting)
What microscope is used on the UTA campus?
Brightfield
Robert Hooke
What light interaction is where the wave is captured?
absorbance
1. heat fix smear
2. primary stain (malachite green)
3. Decolorizer (water)
4. Counter stain (safranin)
what microscope is this?
horizontally, the intensity of the current is measured, and the specimens are coated in conducting metals (like DNA)
Scanning Tunnel Microscope
less frequency means...
less energy
Constructive interference =
What is the difference between a positive and negative stain?
positive (+) stains into the cell
negative (-) stains into the background
What microscope gives a 3D appearance?
Differential interference contrast
about 400-750 nm
refractive index
What is the order of a gram stain?
1. heat fix smear
2. primary stain (crystal violet)
3. mordant (iodine)
4. decolorizer (alcohol)
5. counter stain (safranin)
What are the 6 different microscopes that can use a DEAD specimen?
Brightfield, Fluorescence, Confocal, TEM, SEM, and AFM