Chapter 6

Replication Forks

100

Is the replication fork asymmetrical or symmetrical?

What is assymetrical?

200

This strand goes in the direction of the fork.

What is the leading strand?

300

This strand goes in the opposite of the replication strand.

What is the lagging strand?

400

I have 2000 replication forks, how many replication bubbles are there?

What is 1000?

500

What prime end is DNA built on?

What is the 3 prime end?

Experimental

100

I discovered the transformation principle

Who is Griffith?

200

We used a blender to see that DNA is nuclear in nature.

Who is Hershey and Chase?

300

We found that DNA is transformative, not RNA. Name the experiment.

What is the Avery experiment?

400

Protein contains carbon, hydrogen, oxygen and nitrogen. So does DNA...what are the two elements that differentiate a protein and DNA?

What is Phosphorous in DNA and Sulfur in Protein?

500

We have the MOST beautiful experiment in biology.

What is the Meselson and Stahl Experiment?

Protein Players

100

I add nucleotides to the 3 prime end of a growing strand of DNA, and use the parental strand as a template?

What is DNA polymerase?

200

I use ATP to unwind the DNA

What is helicase?

300

I add RNA primers to Okazaki Fragments

What is Primase?

400

I put together the Okazaki Fragments.

What is DNA ligase?

500

I create nicks in the DNA structure to allow for the DNA to not twist up, relieves tension before helicase comes to unwind the DNA.

What is DNA topoisomerase?

Potpourri

100

UV radiation can create kinks in the DNA in what two nucleotides?

What is C and T?

200

What has the editing ability in DNA replication?

What is DNA polymerase?

300

In the Hershey Chase Experiment, we aimed to see is viral genetic material DNA or Protein. Describe this experiment.

Labeled DNA with 32 P and Protein with 35 S into viruses. Then allowed to infect E. coli. Then the viral heads were shaved off using a blender. After, the pellet formed showed 32 P and not 35 S.

400

Is DNA semiconservative, dispersive, or conservative in replication?

What is semiconservative?

500

Why is semi conservative replication the best option for DNA replication?

Leaves the daughter strand and the parental strand intact. Easier for the cells to do and allows for less mistakes.

Call Home Depot...we need repairs

100

What is DNA Mismatch Repair Systems?

What is removing replication errors that escaped proofreading?

200

Homologous Recombination occurs when?

What is in G2 and S phase?

300

Non homologous end joining happens when?

What is all throughout the cell cycle, mostly G1?

400

Which is better NHEJ or Homologous Recombination?

What is homologous recombination?

500

The proofreading protein is called....

What is DNA polymerase!