Macroenvironment
What does "macro" mean?
Large
Between 6-8
How much does a gallon of water weigh?
8.34 lbs
3.78 kg
What is bioexclusion?
A barrier prevents the entry of all infectious agents from getting into a room.
What is a quarantine area?
Separates incoming animals of unknown health status from animals already housed in the facility
What is the acceptable range of humidity in an animal room?
30-70%
What 2 substances are added to water to disinfect?
Chlorine or chloramine
What are the biggest concerns about aquatic rooms? (4)
- Weight of tanks
- Risk of flooding
- Electrical hazards
- Corrosiveness of salts that are added to the water
What is biocontainment?
Prevents pathogens present in the room from being released into the environment or into adjacent animal populations
Why are procedures done in a procedure room and not an animal room?
They may stress the other animals housed in the room and some procedures need sterile conditions
What temperature should a rodent breeding room be?
72F
22C
What is water hardness?
The measure of the calcium and magnesium ions present in the water
What 3 designs do each aquatic room need?
Drain with mesh coverings
Floor sloped towards the drains
Electrical outlets several feet above floor
What kind of pressure does a bioexclusion room need?
(+ or -)
Positive
What is the minimum measurement food and water must be apart from the wall?
6 inches
What rate does the Guide recommend for room ventilation?
10-15 exchanges of fresh air per hour
Ammonia
Nitrite & nitrate
What is a static system?
Water is only added to maintain an appropriate volume
Ex: Pond, fish bowl
T/F: All equipment, feed, and bedding may have to be sterilized prior to exiting a biocontainment barrier area.
True
Aseptic Room
What is the difference between positive and negative pressure ventilation?
BONUS:
Which pressure ventilation is needed for surgical suites and which pressure is needed for quarantine rooms?
Positive: More air is pumped into the room than is pumped out - raises air pressure, air flows towards the hallway.
Negative: more air is pumped out of the room than pumped in - air from hallway flows into the room
BONUS: Surgical- positive Quarantine- negative
What can chlorine toxicity cause for fish?
Gill necrosis and death by asphyxiation
What is the order of each filter in the recirculating system?
Hint: There are 4 filters, 7 steps
Incoming water - 1st Mechanical filter - Bio-filter - 2nd mechanical filter - carbon filter - UV - Evaporation
What does 'barrier' imply for lab animal scientists?
Implies the use of specialized housing and strict handling and husbandry procedures
Ex) microisolation-type cages or individually ventilated caging
What should NOT be stored in the same room as a feed storage room?
Animals
Detergents
Cleaning Supplies
Drugs
Chemicals