Media
Gram-positive
Gram-negative
Tests
Gram Stain
100

What selective and enriched media is used to isolate Neisseria gonorrhoeae?

Gonococcal media (Thayer martin, Martin lewis, New York City media)

100

What test differentiates staphylococcus from streptococcus species?

Catalase

100

What test can differentiate Enterobacteriaceae from Vibrio?

Oxidase

100

What color is a positive PYR test?

Pink

100

Moraxella catarrhalis is a _______ on gram stain?

gram-negative diplococci

200

What routine media is selective and differential for non-fastidious gram-negative bacilli?

MacConkey agar

200

What tests can diffferentiate Streptococcus pneumoniae from Viridans streptococci?

Bile solubility and Optochin susceptibility

200

What is the TSI reaction for Enterobacteriaceae?

A/A or K/A

200

That is the TSI reaction for Salmonella?

K/A, H2S+, gas+

200
What is the typical arrangement of staphylococci on gram stain?

Gram positive cocci in clusters

300

What media is used to isolate Vibrio species?

TCBS

300

What organism is a aerobic spore forming gram-positive bacilli?

Bacillus species

300

What color are non-lactose fermenting colonies on MacConkey agar?

Clear

300

What is the spot indole reaction for E coli?

Positive

300

What is the gram stain for Campylobacter jejuni?

Gram-negative bacilli - thin, resembling seagulls

400

What routine media will support the growth of Haemophilus influenzae?

Chocolate agar

400

Streptococcus pyogenes is _____ hemolytic.

Beta

400

Which gram-negative organism will swarm on sheep blood agar?

Proteus

400

What is the glucose utilization for Neisseria meningitidis?

Glucose and Maltose

400

What is the typical gram stain for corynebacterium species?

Gram-positive bacilli palisading resembling chinese letters or "V" and "L" shapes

500

What media is used to demonstrate lecithinase production?

Egg yolk agar

500

What test differentiates Staphylococcus aureus from other staphylococci?

Coagulase

500

What gram-negative bacilli has a metallic sheen on sheep blood agar and has a sweet fruity smell like grape koolaide?

Pseudomonas aeruginosa

500

What is the motility pattern for Listeria in motility agar?

Umbrella shaped or inverted Christmas tree

500

What is the typical gram stain morphology for Haemophilus influenzae?

Gram-negative coccobacilli