Lab Procedures
When making a batch, which well is kept blank?
A2
Which log book do we have to fill out every day?
The temperature log
Around what temperature should the lysis warmer be at?
80.0
What can you make by mixing bleach and lysis buffer?
Cyanide
What do you do if you have a first time invalid?
Add an RP code, write that it was a first time invalid on the report, and make sure you pull it an place it in the invalid rerun rack
What control is added during extraction (same times as samples)?
HSC
If you forgot to fill out a date on log, what do you do?
CAR will have to be written for empty spaces, never back fill
What do we do every morning to prep JANUS?
Check temp., humidity, empty waste, fill JANUS with fresh DI water, and clean the tips
How much ethanol is used during the intensive cleaning?
1L
What do we do with the elution plate after it comes off the chemagic?
Give it to whose on JANUS or put it in the JANUS fridge.
When doing a QuikSal STAT on the mini-Cepheid's, what thing(s) can you report out?
SARS-CoV-2
When you reject a specimen during processing or login, what information do you fill out?
Approximately how long an ABI take to run a QuikSal?
1.5 hours
Which machines requires lubrication every month?
The centrifuges
What do you do if you don't know the answer to something?
Look in SOP, ask a manager, ask a senior analyst
What are the names of the two assays we use in our current work flow?
PKamp and QuikSal
Where can you find the monthly and weekly maintenance logs?
The fridge near the batching computers
How many chemagics do we have?
7
How many environments total do we do each month?
15 (5 PKamp, 5 QuikSal, 5 WasteWater)
What reagent can we not vortex?
Enzyme
The PKamp test can test for which 5 things?
Sars-CoV-2, influenza A and B, RSV, and RNase P
When you freeze positives, what log do you need to fill out?
Frozen Positives Log 2021
What is the name of the new liquid handler we are getting?
Biomek i7
What do we use for the monthly maintenance on JANUS?
10% Lysol dilution
Where do we store the SC2PC positive control for QuikSal?
The refrigerator (2-8 degrees C)