SPECIMEN
PROCESSING
PROCESSING
For which of the following reasons should a blood specimen be centrifuged only one time?
a) Centrifuging more than once can result in the specimen being exposed to light.
b) Centrifuging more than once can cause the loss of CO2 and increase the pH of the specimen
c) Centrifuging more than once can lead to hemolysis and inaccurate test results
d) Centrifuging more than once can cause the specimen to clot.
C) Centrifuging more than once can lead to hemolysis and inaccurate test results
Which blood test below would be used to evaluate respiratory function?
a) CBC
b) TSH
c) ABG
d) INR
C) ABG
Which one below is not a rejection criterion for a specimen:
a) delay in testing
b) inverting sodium citrate 3-4 times
c) exposure to light
d) wrong collection time
b) inverting sodium citrate 3-4 times
You have to obtain a Molecular Test specimen. Which tube would be used for molecular testing of hepatitis c qualitative RNA?
a) SST
b) PPT
c) PST
d) RST
B) PPT
Which test below is sent to the serology department for processing and testing?
a) Rubella Titers
b) aPTT
c) Hgb A1c
d) FBS
A) Rubella Titers
Two-part questions
1) Which of the following explains the role of a certified phlebotomist in a clinical setting?
a) collect, prepare, and transport specimens
b) crossmatch blood specimens for transfusions
c) relay test results to patients
d) interpret the relay test results
2) Which of the following is the recommended storage temperature for the majority of specimens during transport?
a) 25 degree Celsius (77F)
b) 4 degree Celsius (39.2F)
c) 37 degree Celsius (98.6F)
d) 50 degree Celsius (122F)
1-A) collect, prepare and transport specimens
2- A) 25 degree Celsius (77F)
2 Questions:
1) Which tube below will be chosen for a STAT chemistry test
a) SST
b) EDTA
c) Light Blue
d) PST
2) Which specimen below is not to be protected from light:
a) Vitamin B6
b) Pyruvate
c) Folate
d) Urine Porphobilinogen
1) D- PST
2) B- Pyruvate
2- Part Questions:
1) Which test below does not commonly appear on a Panic Value list:
a) Na+
b) WBC
c) Ethanol
d) APTT
c) ETOH
d) APTT
2) Which of the following is the purpose ousing a heel w
1) C-ETOH
2) Ethanol
2-PART QUESTION:
1) Which specimen below is not to be chilled in ice slurry or cooling rack?
a) Lactic acid
b) Gastrin
c) Cryoglobulin
d) Ammonia
2) Which of the following would cause a chemistry specimen to be rejected for testing?
a) insufficient labeling
b) Hemolysis
c) Clotting
d) Placing in a biohazard bag
1) C- Cryoglobulin
2) B- Hemolysis
2-Part Question:
1) When collecting blood on filter paper for metabolic syndrome testing, which of the following should be done with the first drop of blood?
a) It should be used to fill the first circle on the filter paper
b) It should be tested immediately with glucose meter
c) It should be sabed for additional testing if needed
d) It should be wiped away before collecting the samples
2) Which of the following is an autologous blood donation?
a) A donation where a person donates blood for the general population.
b) A donation where a person donates blood for their own future use.
c) A donation where blood is taken during surgery and kept for transfusion.
d) A donation where blood is collected and used immediately
1) D-It should be wiped away before collecting the sample.
2) B- A donation where a person donates blood for their own future use.
Two-Part Questions:
1) Which specimen below can be analyzed without being centrifuged?
a) STAT k+/Heparin gel tube
b) Chol/SST
c) Hgb A1c/EDTA tube
d) Bili/amber microtube
2) A serum aliquot has a fibrin clot in it. This occurred because the specimen:
a) spun too long with the tube topper off
b) from pt with a high platelet count
c) hemolyzed from improper transport
d) centrifuged before clotting was complete
1) C-Hgb A1c
2) D- centrifuged before clotting was complete
3 Part Questions:
1) Which of the following specimens can be centrifuged immediately:
a) Electrolyze/PST
b) Bili/Serology Tube
c) CBC/EDTA tube
d) Creatinine/SST Tube
2) Which of the following tests is often impacted by light?
a) Troponin
b) Ammonia
c) Bilirubin
d) Glucose
3) After obtaining a specimen for a cold agglutinin test, the blood must be transported:
a) protected from light
b) at Body temperature
c) in a cooling rack
d) as a STAT test
1) A- Electrolyte/PST
2) C- Bilirubin
3) B- at Body temperature
3-Part Questions:
1) Which of the following blood specimens requires cooling during transportation?
a) Beta-carotene
b) Electrolytes
c) Homocysteine
d) Cold agglutinin
2) Which specimen collected below would be affected by the PTS transport?
a) AST
b) TT
c) BUN
d) Cryoglobulin
3) After you have obtained a specimen for a cold agglutinin test, the blood must be transported:
a) protected from light
b) body temperature
c) a cooling rack
d) as a STAT test
1) C- Homocysteine
2) D- Cryoglobulin
3) B- Body temperature
3-Part Question:
1) A splash of serum is released when the stopper of a centrifuged serum separator tube is removed. What is the most likely reason?
a) the centrifugation time was too long
b) the tube was titled after it was centrifuged
c) the separator gel worked incorrectly
d) the stopper was removed too quickly
2) During the Preexamination/Preanalytical stage, errors can occur at the time of collection; which one below is not during the , "St time of collection", phase:
a) wrong collection time
b) incorrect collection tube
c) antiseptic not dry
d) agitation-induced hemolysis
3) Which specimen should not be centrifuged?
a) CBC
b) Protime
c) Glucose
d) Vitamin B12
1) B- the tube was titled after it was centrifuged
2) D- agitation-induced hemolysis
3) A- CBC
2-Part Question:
1) Which of the following actions is appropriate to take if bleeding from an infant's heel puncture site does not stop after applying pressure?
a) Continue to hold pressure until the bleeding stops, then bandage the site
b) Immediately call for a nurse to address the bleeding
c) Leave the site unbandaged and place the infant back in their crib
d) Use a tourniquet to stop the bleeding
2) Which of the following is the purpose of the read-back process when reporting critical values from point-of-care testing (POCT)?
a) To ensure a pt. understands their test results
b) To train new staff on how to communicate test results
c) To provide a written record of the conversation for legal purposes
d) To confirm the accuracy of the information with the person receiving the results
1) A -Continue to hold pressure until the bleeding stops, then bandage the site
2) D- To confirm the accuracy of the information with the person receiving the results
Two-Part Questions:
1) Which of the following is the clot time of a specimen prior to centrifuging?
a) 30 min.
b) 5 min.
c) 15 min.
d) 90 min.
2) Which of the following is the purpose of an air waybill in the context of clinical research specimen transport?
a) It acts as a shipping label and tracking form with details about the sender, recipient and special shipping instructions.
b) It is a detailed report of the phlebotomist's performance during the specimen collection
c) It serves as consent form for the pt. whose specimen is being transported
d) It is used to record the pt's medical history and previous test results
3) Which specimen below does not need to be protected from light?
a) Beta-carotene
b) Folate
c) Parathyroid hormone (PTH)
d) Vitamin C
1) A- 30 min.
2) A- It acts as a shipping label and tracking form with details about the sender, recipient and special shipping instructions
3) C- Parathyroid hormone (PTH)
3- Part Question
1) Drawing this tube before a chemistry tube can result in false hypocalcemia:
a) SST
b) PST
c) EDTA
d) Heparin
2)Glucose specimens are room temperature and drawn in a sodium fluoride tube are stable for:
a) 24 hours
b) 8 hours
c) 36 hours
d) 48 hours
3) Which blood test below would be used determine if a pt has Leukemia:
a) PTT
b) CBC
c) TnT
d) CRP
1) C- EDTA
2) A- 24 hours
3) B- CBC
3- Part Question:
1) Which of the following would be correct procedure for thawing a frozen sample:
a) warm it at 25 degree Celsius; invert 10-20 times after thawing
b) thaw it in the refrigerator; mix only when thawed
c) warm in at 37 degree Celsius; mixing it when ready for testing
d) heat in a warm water bath; invert it occasionally
2) As a general rule, serum or plasma should be removed from the cells within:
a) 30 min.
b) 60 min.
c) 90 min.
d) 120 min.
3) According to CLSI, which test is negatively affected by tube system transportation?
a) Creatine
b) Albumin
c) K+
d) Uric Acid
1) A- warm it at 25 degree Celsius; invert 10-20 times after thawing
2) D- 120 min.
3) C- K+ (Potassium)
3-Part Question:
1) The specialized application of digital information technology to optimize lab operations is called:
a) networking
b) electronics
c) computerization
d) informatics
2) Which of the following temperatures is acceptable for a specimen that requires transportation and handling at room temperature?
a) 25 degree C
b) -20 degree C
c) 8 degree C
d) 37 degree C
3) Latent fibrin formation in serum can result from
a) a centrifuge speed that is too high
b) incomplete clotting when centrifuged
c) gross hemolysis of specimen
d) long delay before specimen was centrifuged
1) D- informatics
2) A- 25 degree C
3) B- incomplete clotting when centrifuged
2 Part Question:
1) Place the steps for actions to take in a fire in the correct order
A. Confine the fire by closing doors and windows
B. Activate the fire alarm
C. Rescue others who are in danger
D. Extinguish the fire with the nearest fire extinguisher
2) Which of the following is the purpose of balancing tubes in a centrifuge?
a) To prevent damage to the centrifuge
b) to mic the contents of the tubes
c)To increase the speed of the centrifuge
d) To reduce the noise produced by the centrifuge
1. C, B, A, D
2) A- to prevent damage to the centrifuge
3- Part Question:
1) After arriving in the lab, all specimens are immediately:
a) refrigerated until processed
b) uploaded to testing instruments
c) logged in or accessioned
d) centrifuged to stop glycolysis
2) Which one below would not be found commonly on a Panic Value lists:
a) Hgb
b) FTA-ABS
c) PT/INR
d) Bilirubin
3) Inadequate mixing of an anticoagulant tube can lead to:
a) cell decomposition
b) extreme hemolysis
c) microclot formation
d) immediate glycolysis
1) C-logged in or accessioned
2) B- FTA-ABS
3) C- microclot formation
3 PART QUESTIONS:
1) Which specimen below is not to be chilled in ice slurry or cooling rack:
a) Lactic Acid
b) Fastrin
c) Ammonia
d) Cryoglobulin
2)Your pt presents for a bilirubin test. Which drug therapy below can increase bilirubin levels?
a) Chemotherapy
b) Acetaminophen pills
c)Birth control pills
d) Thiazide diuretics
3) Which of the following does the mnemonic code "5mL LAV" indicate to a phlebotomist that:
a) the amount of blood and the color of the tube needed for a test
b) the type and amount of medication to be administered
c) the pt.'s billing information
d) the pt.'s diagnosis
1)D- Cryoglobulin
2) B- acetaminophen pills
3- Part Question:
1) A blood specimen aliquot can remain at room temperature for no longer than:
a) 1/2 hour
b) 2 hour
c) 6 hour
d) 8 hour
2) All of the following are required PPE when processing specimens except:
a) fluid-resistant shoe covers
b) chin-length face shields
c) disposable gloves
d) fluid-resistant lab coats
3) Which federal agencies regulate the transporting of biological specimens off-site?
a) WHO & OSHA
b) CLIA & EPA
c) DOT & IATA
d) EPA & CLSI
1) D- 8 hour
2) A- fluid- resistant shoe covers
3) DOT & IATA
2-Part Question:
1) When collecting blood on filter paper for metabolic syndrome testing in infants, which of the following is a recommended practice?
a) Fill the circles on the filter paper by smearing the blood with a gloved finger
b) Allow the filer paper to absorb the blood from both sides to ensure complete saturation.
c) Fill the circles on the filter paper by touching the paper directly to the blood drop
d) Send the filter paper to the laboratory immediately after blood collection, without waiting for it to dry
2) Which of the following is the purpose of a glucose tolerance test (GTT)?
a)To measure the pt.'s ability to tolerate glucose in their diet.
b) To determine how well the pt.'s body processes glucose after ingestion.
c) To screen for potential allergies to glucose
d) To confirm the pt.'s blood type
1) C- Fill the circles on the filter paper by touching the paper directly to the blood drop
2) B- To determine how well the pt.'s body processes glucose after ingestion.
2- Part Question:
1) Which of the following explains the significance of the Rh factor in blood transfusions
a) Rh-negative individuals can safely donate Rh-positive blood
b) Rh-positive individuals can sagely donate blood to Rh-negative individuals
c) Rh-negative individuals should only receive Rh-negative blood
d) The Rh factor is unrelated to blood compatibility in transfusions
2) Which of the following chambers of the heart receives oxygen-poor blood from the body?
a) Right Atrium
b) Left Atrium
c) Right Ventricle
d) Left Ventricle
1) C-Rh-negative individuals should only receive Rh-negative blood.
2) A- Right Atrium