Purpose
What is Crispr-Cas9?
Crispr-Cas9 is a gene-editing tool that allows researchers to modify target genomes.
Why do we want to design the enzyme?
To move it to the target site.
What test was Crispr-Cas9 successful in making
Coronavirus detection tests
What does Crispr-cas9 prevent?
It prevents DNA integration.
Why is Crispr-Cas9 better than previous tools?
Because it is cheaper and easier to use.
What are the two parts of Crispr-Cas9?
A guide RNA to match the target gene and Cas9, an endoclause.
What do the cells naturally undergo after the double-strand break?
Cells naturally undergo non-homologous end-joining pathways.
What does Crispr-Cas9 allow scientists to do?
It exponentially helps in disease research.
Crispr-cas9 can lead to what down the line?
Mutations.
How do bacteria benefit from Crispr-Cas9?
Bacteria use CRISPR systems to protect themselves from virus attacks.
What does Cas9 do?
It causes a double-stranded DNA break, allowing changes to the genome.
Fill in the blank: For deciding the success of on-target editing and for investigating off-target effects, ___________________ is highly recommended.
For deciding the success of on-target editing and for investigating off-target effects, next-generation sequencing (NGS) is highly recommended.
What diseases were the examples for the research?
The diseases were cancer and various mental illnesses.
True or False: These mutations can be inherited by all future generations.
True
What does Crispr stand for?
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats.