Toxicology Basics
Postmortem Toxicology
Forensic Entomology Basics
Insect Development & PMI Estimation
Advanced Entomology: Succession & Identification
Stages of Decomposition
100

Toxicology is the scientific study of what?

The adverse effects of chemicals on living organisms.

100

What is analyzed during an autopsy to screen for toxins?

Body tissues, organs, and fluids.

100

True or False: The first insects to arrive at a corpse are typically beetles.

False. (It is usually blowflies.)

100

If only blowfly eggs are found, what is the likely PMI?

8–48 hours.

100

Which blowfly life cycle stage is shown in the picture below?

Pupa

100

What is the first stage of decomposition, beginning immediately after death?

Fresh

200

What does forensic toxicology help determine?

Cause, mechanism, and manner of death.

200

After detecting a toxin, what must a toxicologist determine?

The type of toxin present.

200

True or False: Larvae go through three instars before becoming pupae.

True

200

What insects arrive first at a corpse?

Blowflies. Within minutes to hours

200

Which blowfly stage is shown in the picture below?

Instar II

200

In which stage does the body swell due to gas buildup from bacterial activity?

Bloat

300

What does a toxicologist do with biological samples?

Detects, identifies, and measures drugs, poisons, and toxins.

300

After identifying the toxin, what is measured next?

The concentration/amount.

300

Which stage comes after larva in the blowfly life cycle?

Pupa

300

What insects dominate during advanced decay?

Dermestid beetles and rove beetles

300

Flesh flies and beetles begin to appear during which stage?

Bloat

300

In which stage do maggot masses actively feed and cause rapid tissue loss?

Active decay

400

What are the four stages a chemical undergoes in the body?

Absorption, distribution, metabolism, elimination.

400

What type of equipment is used to detect small amounts of toxins?

Analytical and instrumental equipment.

400

True or False: Maggot mass temperature can be significantly higher than ambient temperature.

True

400

Presence of pupae (tan/brown casings) suggests a PMI of…

5-10 days

400

Which stage is when blowflies are MOST active in feeding?

Larval (instar) stage, especially instar III

400

What stage is characterized by reduced insect activity and drying of remaining tissue?

Advanced decay

500

What does LD50 represent?

The dose that kills 50% of test organisms.

500

Why are metabolites useful in toxicology?

They show how a drug was processed by the body.

500

Why are maggots useful for estimating PMI?

Their development is predictable under known conditions.

500

Second instar larvae on the body indicate a PMI of…

2-3 Days

500

A body is found with both adult dermestid beetles and pupating blowflies (in the pupa stage of development). Ambient temperatures have been normal. Based on typical insect succession, what can you infer about the stage of decomposition and PMI?

advanced decay; 2-3 weeks

500

What is the final stage where only bones, hair, and dried tissue remain?

Dry remains/skeletal remains