cold reactive autoantibodies
for cold reacting autoantibodies , what DAT result would you get if you are using IgG monospecific AHG reagent ?
Negative
The most common antibody seen to cause WAIHA is
anti-e
Large doses of Penicillin can cause which type of Drug induced +DAT?
Drug absorption - Drug sticks to the RBC and body makes an antibody to the drug
a patient who has a mixed field reaction with all adult human serum most likely has
Tn polyagglutination ( Transient Aberrant Erythropoeisis ) - this is a mutation of hematopoetic tissue
An antibody reacts with 1 in 10 random test cells in the antiglobulin phase. Reactivity is not affected by enzymes, but is destroyed by AET. The antibody is most likely:
Anti-K
The most common cause of cold reacting autoantibody is
anti-I
We can get false positive testing for D testing when doing weak D test in patients with WAIHA , what will let us know this is a false positive ?
The D control will be positive
Which drug can cause Immune- Complexes to be formed which attached to the RBC activating complement
If a patient issues with polyagglutination when testing patient cells with antisera what can you do to get rid of these reaction?
Test with monoclonal reagent
What is strongly suspected if you have mixed field and refractile agglutinations
Anti- Sda
What technique is used to investigate cold autoantibodies?
pre-warm technique
What type of hemolysis is seen with WAIHA?
extravascular
Methyldopa and Aldomet can cause a postivie DAT by which mechanism?
Auto-antibody formation
Can a patient with polyagglutination donate blood ?
No
A patient needs 6 units of K neg RBCs. How many units will you likely need to screen?
The antibody responsible for CHD ( cold hemagglutinin disease ) is
anti-I
When performing an autoadsorption on patient with WAIHA what is the first step?
treat patient cells to strip antibody off of them, this will allow us to use those cells to pull autoantibody out of the plasma.
in Membrane modification mechanism of + DAT caused by cephalosporins , the eluate tested will give what type of reaction?
Negative
What type of reactions would you get when mixing a patient with polyagglutination with cord serum?
negative
A positive DAT may be caused by any of the following except:
a. Autoanti‑c
b. Baby with HDN due to anti‑A,B
c. Aldomet
d. Anti-Lua
D
The antibody responsible for PCH is
anti-P
If a patient with a warm autoantibody also has underlying alloantibodies we must identify the alloantibodies. It may be difficult because the patient will have a positive DAT so antigen typing the red cells is hard. What can we treat the Red cells with to remove the IgG and leave cells intact?
Chloroquine
If positive DAT is caused by Drub absorption , what will the result of the antibody screen likely be?
Negative - unless you are using drug coated cells or patient has an alloantibody
Can Tn polyagglutination go away ?
No - it is permanent and irreversible
Anti‑i
a. may be the cause of hemolytic episodes in patients with infectious mononucleosis
b. Very common in the general population
c. is seen in people with paroxymal cold hemoglobinuria
d. seen primarily on adult cells
a