Developmental regulation of tau splicing is disrupted in stem cell-derived neurons from frontotemporal dementia patients with the 10 + 16 splice-site mutation in MAPT

Alzheimer's

100

Alzheimer's is the neurodegeneration of what

What is Brain Cells

200

Alzheimer's affects cognition and what

What is behavior

300

Alzheimer's currently has no what

What is Treatment/Cure

400

Alzheimer's is incurable due to the lack of understanding of what

What is mechanisms

500

Alzheimer's will affect what number of people by 2050

What is 100 million

Tau Splicing

100

MAPT splices into what number of isoforms

What is 6 Isoforms: 0N3R, 0N4R, 1N3R, 1N4R, 2N3R and 2N4R 

200

These 2 isoforms are present in equal amounts

What is 3R and 4R isoforms

300

Tau is healthy in the adult brain until it begins to form what

What is Tau Plaques

400

Tau Plaques degrade what

What is healthy neurons

500

Most tau splicing mutations occur at what

What is exon 10

iPSCs

100

iPSCs were created from what

What is fibroblasts

200

Fibroblasts were transduced using what viral 

What is cMyc, Oct4, Sox2, and Klf4

300

iPSCs were differentiated into what

What is neurons 

400

iPSC derived neurons express what

What is 0N3R tau

500

iPSCs have the potential to model what

What is FTD

exon 10

100

FTD patients have a what mutation

What is 10 + 16 site mutation

200

10 + 16 mutation destabilizes what

What is stem loop 

300

exon 10 increase can override what

What is regulation of tau splicing

400

after 100 days, iPSCs with 10+ 16 mutation showed what

What is 4R isoforms, which were lacking from control cells

500

After 365 days, 10 + 16 iPSCs expressed higher forms of what than control cells

What is 4R isoforms