Deamination of C
What is the product of Cytosine deamination?
Uracil
product of Adenine deamination is?
hypoxanthine (HX)
What number and which atoms of which nucleic acids are targets of alkylation?
N7 of Guanine, and N3 of Adenine. (also phosphate)
Which reactive oxygen species is the most reactive?
hydroxyl radical
what causes dimerization?
ultraviolet light
Guanine
HX prefers H-bond with which nucleic acid?
Cytosine (C)
what does N7 alkylation of Guanine result in?
spontaneous depurination and the formation of an AP site
what generates hydroxyl radical?
H2O2
which nucleic acids dimerize?
pyrimidines, C and T
U:A base pair is favored due to this geometry.
watson crick
What is the resulting mutation?
A to G
What is preferentially incorporated opposite an AP site?
adenine
What does 8-oxoguanine preferentially bind to?
Adenine
What repairs dimerized nucleotides?
NER, Nucleotide excision DNA repair
what enzyme removes uracil?
Uracil DNA Glycosylase
It is removed by which enzyme?
alkyladenine DNA glycosylase
O6-alkylation of Guanine result in which kind of mutation?
G-to-A mutation
what mutation does oxoguanine result in?
G-to-T mutation
Further processed downstream by?
BER (base excision repair proteins)
further repaired by?
BER proteins
what else can result from alkylation of O6-methylguanine?
DNA termination and strand breaks