DNA Facts
This is what DNA stands for.
deoxy-ribonucleic acid
The three factors (in order) that stabilize dsDNA.
What are H-bonding, ionic bonding, and bp-stacking forces?
This is what happens when DNA is in a solution with a very high pH?
What is "denaturation?"
Every other quantification method we talked about this week requires a standard curve OTHER than these two?
What are spectrophotometry and digitial PCR?
The three steps that Jaime and Gunther talked about for purifying DNA with either silica membranes or magnetic beads.
What are "Bind, Wash, Elute?"
The property of DNA that makes it soluble in water (and makes electrophoresis possible).
What is Polarity?
A property that is affected by how stable the dsDNA molecule is.
What is melting temperature?
This is what happens when DNA is in a solution with a low pH?
What is "depurination?"
X-rays, microwaves, WiFi, radio waves, and the color blue.
What are some different wavelengths of light?
An upgrade to a quant kit (or STR amp kit) that allows the probe to be shorter while still binding at the proper temperature.
What is the functional group of a TaqManMGB probe?
These bases have two conjugated rings of carbon atoms.
What are the purines adenine and guanine?
These two instruments first create lysate and then actually extract the DNA for BFS casework units.
What is the Hamilton AutoLys and the Kingfisher Flex instruments?
This blocks the DNA polymerase from extending the DNA strand during PCR.
What is pyrimidine dimerization caused by photodegradation from UV light?
Represents how well a change in fluorescence is explained by a change in concentration.
What is the r-squared value of your standard curve?
This is one of the disadvantages of qPCR (as opposed to some other methods we learned about).
Subject to inhibition, small variations in Ct lead to big changes in quantity, quantifies specific targets not amplifiable DNA, low precision at low copy number, assumes samples amp at same efficiency as standards...
The two proteins that DNA wraps around in order to pack it into a cell efficiently.
What are histones and protamine?
The exact name of the extraction kit used by BFS's Missing Persons unit on the Automate Express.
What is the PrepfilerBTA kit?
An enzyme that causes the hydrolysis of the sugar-phosphate backbone in DNA.
What is a "DNase?"
This is the downward effect of a lowered slope on your standard curve.
What is "your DNA concentration will be overestimated, which will cause your STR typing results to be partial (missing peaks)?"
Who is Rosalind Franklin?
The name of the nucleotide that contains an "A" base.
What is Adenosine (actually Deoxyadenosine monophosphate)?
These make up 90% of this evidence type found at crime scenes.
What are shed hairs?
The two components of TE and their purposes.
What is tris (buffers the pH) and EDTA (chelates positive metal ions that serve as cofactors for DNases).
This is what happens to light when it is absorbed by a substance and then re-emitted.
What is "the wavelength gets longer (and frequency gets lower)."
The thing Eric harped on the MOST this entire week.
What is "Quant dilutions?"