DNA History
Rosalind Franklin discovered that DNA was a ________ width.
Uniform/Consistent
Where does Transcription occur?
In the Nucleus
In Gel Electrophoresis, the _____________ fragments of DNA go the furthest and fastest.
Smallest
This is the phase of the cell cycle that DNA is replicated in.
S or Synthesis Phase
The liquid poured into the electrophoresis tank is called what?
Buffer
In Griffith's experiment using bacteria & mice, he discovered what he called a "_________________ _______________________".
A "transforming principal"
During Transcription, DNA is read to create what?
RNA
In electrophoresis, what molecule type of traveling through the gel?
DNA
This type of molecule does all of the "work" to replicate DNA.
Proteins or Enzymes
In electrophoresis, DNA moves towards the positive pole because it holds what kind of charge?
Negative
Oswald Avery built on Griffith's experiment, and determined that the transforming principal was what molecule?
DNA
Where does Translation occur?
In the cytoplasm
During electrophoresis, DNA moves from the ______ pole to the _______ positive pole.
negative pole to the positive pole
This is the monomer of nucleic acids.
Nucleotide
If you leave your electrophoresis gel run too long, what will happen to the DNA?
It will run off the end of the gel.
Watson & Crick determined the shape of DNA by doing what?
Building 3D models.
What is the name for the 3 letter sequence of bases in mRNA that is read to make proteins?
Codons
In Gel Electrophoresis, the largest fragments of DNA will be where on the gel in relation to the well that it was loaded into?
Closest/Closer to the well
This is the name for the type of replication DNA goes through where one new strand is built around an original parent strand.
Semi-conservative replication
What can happen if you don't change pipette tips in between loading samples into the gel?
Contamination or cross over of samples
Erwin Chargaff analyzed the DNA of various organisms, what did he conclude?
A & T = %
C & G = %
During Translation, mRNA is read to create what?
Proteins
This is the precise measuring instrument that is adjusted and used to load DNA samples into the wells.
Micropipette
Name the 3 parts of a nucleotide.
Phosphate group, sugar, nitrogen base
Name the person and time period that they created the lab practice of using gel electrophoresis?
Arne Tiselius in the 1930's