Proposed Models of DNA Replication
In this model of replication, one old DNA strand combines with one new DNA.
Semiconservative replication
This enzyme unwinds the DNA double helix at the replication fork.
Helicase
How are fragments separated by gel electrophoresis?
By length
This strand is synthesized continuously.
Leading strand
In this model of replication, the original DNA strands reform the original double helix, while the two new DNA strands form a new double helix.
Conservative replication
This enzyme adds DNA nucleotides to the 3' end of an elongating strand.
DNA polymerase III
Which four US states begin with the word "New"?
New Hampshire, New Jersey, New Mexico and New York
This enzyme adds a short strand of RNA nucleotides known as a primer.
Primase
How were bacterial strands of DNA marked/differentiated to discover the method of DNA replication?
isotopes of nitrogen (light and heavy)
What city do Batman and Robin patrol?
Gotham City
What is used to cut DNA samples for analysis?
Restriction enzymes
Who wrote "James and the Giant Peach"?
Roald Dahl
What famous blind musician has the real given last name Judkins?
Stevie Wonder
This enzyme relieves supercoiling ahead of the replication fork.
DNA gyrase
What enzyme is used in PCR?
taq polymerase
The pieces of DNA made on the lagging strand
Okazaki fragments
In this model of replication, the original DNA strands break into pieces and the new DNA reassembles as a mixture of old and new DNA.
100% Hybrid models.
Dispersive replication
This enzyme seals the fragments at the end of replication.
DNA ligase
What are the 3 steps of PCR?
Denature, anneal, sythesize
Which strand requires RNA primers
Both leading and lagging strands