Two repair strategies for double-stranded breaks
What is non homologous end joining and homologous recombination?
This is the enzyme that adds new nucleotide bases to the parent or template strand to form a new DNA strand
What is DNA Polymerase
The spontaneous loss of an amino group from cytosine to produce uracil
What is deamination?
Uses Helicase, DNA Polymerase, Nuclease, and DNA ligase
What is the lagging strand
Two catalytic domains of DNA polymerase
What are the polymerizing and editing domains?
Forms a phosphodiester bond between the 5’ phosphate of one DNA fragment with the adjacent 3’ OH of the next
What is DNA Ligase?
The first step to DNA damage repair
What is Damaged DNA is recognized and removed by nucleases
Copies in the 3' to the 5' direction
What is the lagging strand?
Model of DNA replication where parental and newly synthesized strands become randomly mixed during the replication process
What is dispersive DNA replication?
RNA polymerase that synthesizes RNA primer required for initiation of DNA replication
What is Primase?
100 to 2000 nucleotide fragments formed in discontinuous replication
What are Okazaki fragments?
The second step to DNA damage repair
Repair DNA polymerase binds free 3’ OH of cut DNA strand – fills gap
Produces breaks in the DNA molecules to prevent supercoiling and knot formation during replication, then rejoins
What is topoisomerase?
Two steps involved in DNA mismatch repair
What is 1. Removal of a portion of DNA strand containing mismatch 2. Resynthesize missing DNA
Covalent linkage between two adjacent pyrimidine bases, exposure to UV light
What are thymine dimers?