Enzymes
Structures
Order of Processes
Leading, Lagging, or Both?
Grab Bag
100
This is the enzyme that "unzips the genes"
What is helicase?
100
This is the word for the shape of DNA
What is a double-helix?
100

Step 1

What is helicase unzips the genes, forms a replication fork (Initiation)

100

Copies from the template strand, moving continuously from 3' to 5' along the strand.

What is the leading strand?

100
The name of the area where DNA begins to replicate/copy/divide
What is the replication fork?
200

This is the enzyme that adds new nucleotide bases to the parent or template strand to form a new DNA strand

What is DNA Polymerase

200
The four nucleotide bases
What are Adenine, Thymine, Guanine, Cytosine?
200

Step 2: Leading Strand

RNA polymerase adds a primer, DNA polymerase adds complimentary bases from 5' to 3' end

200

Uses Helicase, DNA Polymerase, RNA primase, and DNA ligase

What is both?

200
The complimentary strand to a template DNA strand that reads "CAT TAC GCT"
What is "GTA ATG CGA"?
300

This is the enzyme that links all the bases on the lagging strand together

What is DNA Ligase?

300
The partnering of nucleotide bases on DNA ...
What is A=T, G=C
300

Step 3: Lagging strand

RNA primase adds a primer to lagging strand, DNA polymerase builds strand, process repeats

300

Copies in the 3' to the 5' direction in short chunks.

What is the lagging strand?

300

The three parts of a nucleotide?

What are sugar, phosphate and nitrogenous base?

400

The enzyme that replaces the RNA primers on the lagging strand?

What is DNA Polymerase

400
These are the fragments formed on the lagging strand by DNA Polymerase III.
What are Okazaki fragments?
400

Step 4

Okazaki fragments are bound together by DNA ligase, DNA polymerase proofreads

400
Forms Okazaki fragments
What is the lagging strand
400

How can you tell the different between an RNA primer and DNA nucleotides?

What is the presence of Uracil?

500

This adds nucleotide that tells the DNA polymerase to begin adding new bases to the template strand?

What is RNA primase?

500

One difference between RNA (ribonucleic acid) and DNA (DEOXYribonucleic acid)

Number of strands, different sugar, different bases, etc.

500

Final Product?

2 strands "daughter" of DNA from 1 "parent" strand

500
Forms a new "daughter" strand of DNA by using the "parent" strand as a template
What is BOTH!
500

The form the DNA must be in for replication to occur. (Hint: the form that DNA is in during interphase)

What is chromatin?