Experiments
Who is responsible for discovering the structure of DNA?
James Watson & Francis Crick
Honorable mention- Rosalind Franklin
What basses pair together in DNA?
A-T
C-G
What does Helicase do?
Unzips and opens up DNA strand.
Frederic Griffith's Experiment using mice and alive vs killed bacteria that resulted in the mouse either living or dieing indicated what
What charge does DNA have and would large or small fragments move slower?
negative and large
What does Polymerase do?
Add the nucleotide bases in the 5' - 3' direction.
In 1944 who tested DNA, RNA, and Proteins in bacteria to discover the DNA was the transforming agent?
Avery, McCarty, MacLeod
What do we call the we refer to DNA as in relation to the direction of DNA replication? And what direction does DNA get replicated?
Anti Parallel and in the 5' - 3' direction.
What does Ligase do?
Connects/ joins the Okazaki fragments together.
Hershey and Chase conducted an experiment where they radioactively labeled bacteria and DNA, Thus they determined what?
DNA was entering the infected bacteria and must be the genetic material.
What 2 bases are known as purines?
Guanine and Adenine
"Remember CUT the Py"
What does topoisomerase do?
Releases the tension from super coiling that happens as DNA unwinds.
Meselson and Stahl grew bacteria in a heavy isotope and then let it replicate in a much lighter isotope once and then twice. What did the results show?
After 1 replication there was a single band of DNA weighting all the same. After the second replication there were 2 bands, one in the same place and one half way lighter.
If you have 100 basses (50 pairs), and there are 34 cytosine how many adenine are there?
16
What does Telomerase do?
Adds telomeres to protect DNA from getting smaller after replication.