DNA
RNA
Transcription
Translation
Mutations
Electrophoresis
100
The shape of DNA
Double Helix
100
What RNA is copied from
DNA
100
Where it takes place
nucleus
100
where it takes place
ribosome
100
What a mutation is
A change in DNA
100
Restriction enzyme "Z" cuts at AGGC between the to "Gs" (AG|GC). How many DNA fragments will be produced from the DNA strand below?
ATGCGTAATGCCAAGGCTATCTACTAGGAAGGCACTA
3
ATGCGTAATGCCAAG | GCTATCTACTAGGAAG | GCACTA
200
Three parts of a DNA nucleotide
200
Where it is made
nucleus
200
How much of a chromosome is transcribed at one time
One gene
200
What is a codon?
A unit of three nitrogenous bases in mRNA read by the ribosome
200
What kind of mutation?
AGA ->ATA
Point/substitution
200
Which kind of DNA fragments migrate further through the gel? Why?
Smaller fragments move further through the gel because they can move more easily between the pores in the gel.
300
Complete the complementary DNA strand:
ATG CAT GGC
TAC GTA CCG
300
What type of RNA is this?
tRNA
300
Which letter is transcription?
X
300
Name a DNA codon that would result in the amino acid Leu
Many options...
300
What kind of mutation?
Original: TACGGCTAGTCGA
Mutant: TACGCTAGTCGA
Deletion/Frameshift
300
Whose blood is it?
Mike's
400
The enzyme that builds new strands of DNA
DNA polymerase
400
3 types of RNA
mRNA, tRNA, rRNA
400
Why transcription must take place in the nucleus
DNA is too large to leave the nucleus
400
Translate the following RNA sequence
AUG
Met/Methionine
400
What effect this mutation will have:
Original: TAC GCT ATC
Mutant: TAC GCG ATC
None: silent mutation
400
Which is the step child to the father?
Child 2
500
The type of bonds that connect nitrogenous bases
Hydrogen bonds
500
3 structural differences between DNA and RNA
DNA: Thymine, 2 strands, deoxyribose
RNA: Uracil, 1 strand, ribose
500
Transcribe the DNA below:
TAC TGG CGA ACT
AUG ACC GCU UGA
500
What protein sequence will result from this DNA sequence:
GGC ATG TAG
Proline Tyrosine Isoleucine
500
Introduce one substitution to create a nonsense mutation:
TTT ACG GCA
ACG -> ACT
500
Draw an electrophoresis gel based on the DNA fragments below. Who is the daddy rooster?
Chick: 200, 250, 500, 800
Hen: 250, 300, 700, 800
Rooster 1: 200, 250, 800, 900
Rooster 2: 200, 300, 500, 700
Rooster 2