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Structure and Function of DNA
DNA Replication
RNA and Transcription
Protein Synthesis
Mutations
100
One DNA strand has a base sequence GTCCATA. The base sequence on the second strand of DNA is...
CAGGTAT
100
What does the word "replicate" mean? During what phase of the cell cycle does DNA replication take place?
S-phase
100
A single stranded piece of a DNA has the following bases: ACTGAG. What is the matching sequence on its RNA transcript?
UGACUC
100
This process turns mRNA into a protein.
Translation.
100
What is a mutation? Name 2 environmental factors can cause a mutation.
Any change to an organism's DNA/genome. Environmental factors: chemicals, radiation, sun, food, & viruses
200

What are the 3 parts of a nucleotide?

Phosphate, sugar and nitrogen base


200
What is the end product of DNA replication (be very specific!)
Two new molecules of DNA, each is identical to each other and to the original molecule.
200
Transcription: What does is make? What enzyme makes the product? Where does it take place?
RNA. RNA polymerase. Nucleus.
200
What cell organelle makes protein? What are the monomers of protein called?
Ribosome / amino acids
200
What is a frameshift mutation?
This type of mutation occurs when bases are inserted or deletedm, causing ALL the codons from the point of mutation forward to change, thus change causing all the amino acids to change.
300

Describe the function/purpose of DNA in organisms. What is a gene?

A gene is a sequence of DNA nucleotides that acts as a recipe for a specific protein.

300
Name the two enzymes involved in DNA replication and describe their function.
Helicase: unzips/unwinds the DNA helix by breaking the bonds between the bases. DNA polymerase: makes DNA by adding complementary bases to the other side of each of the original strands.
300
Why can’t DNA leave the nucleus?
DNA is too long/bulky to exit through the nuclear pores.
300

What is the start codon in protein synthesis?

AUG

300
Are all mutations harmful? If not, give a counter example.
Helpful mutations: having ONE sickle cell gene makes you immune to the parasite that causes malaria. Neutral mutations: blue eyes
400
These two men are credited with figuring out the shape & structure of DNA. They used a photograph taken by a female fellow scientist (+points if you can name the female scientist).
James Watson and Francis Crick (+points for Rosalind Franklin)
400
Why does DNA need to be copied?
So that when the cell divides, each new cell has a complete set of DNA.
400
Name the three different types of RNA and describe the function of each of them.
mRNA: delivers a copy version of a DNA gene from the nucleus to the ribosome. tRNA: transfers the amino acids to the ribosome. rRNA: part of the ribosome.
400

Translate AUG, GCU, AAA

Met, Ala, Lys

400
Give one example of a harmful mutation and one example of a lethal mutation.
Harmful example: sickle cell anemia, hemophilia, & cancer. Lethal mutation: Tay Sachs Syndrome
500
In a sample of DNA, 40% of the bases are adenine. What percentage of bases are guanine?
10%
500
Why is DNA replication called a "semi-conservative" process?
DNA replication is "semi-conservative" because each new molecule of DNA has one original strand and one newly synthesized strand (it is half conserved, or half saved)
500
Name 4 differences between DNA and RNA.
DNA: deoxyribose, thymine, long, double-stranded, made by DNA replication. RNA: ribose, uracil, short, single-stranded, made by transcription.
500

Translate to protein:

DNA: ATA TGA  CGC

TYR, THR, ARG

500
What is a chromosomal mutation? Give an example.
Alterations to a piece of a chromosome or a whole chromosome [e.g., Down syndrome (3 chromosome #21)]