Bacterial Transformation
This is the strain of harmless E. coli that we used for all of our experiments.
What is MM294?
These what restriction enzymes were originally evolved to fight off.
What are bacteriophage viruses?
This is where plasmids can be found out in the wild.
What is bacteria?
These are used to measure very small volumes of liquid in the lab.
What are Micropipettes?
This is the name of the person who manages the laboratory. She covered the class instruction when Kelly was absent one day.
Who is Allison?
In order for bacteria cells to be considered "competent" they must be trapped in this phase of growth.
What is mid-log growth?
This is the combination of enzymes we used to digest our plasmids.
What is BamHI / HindIII?
The plasmids we worked with all contained resistance to _______________.
This device spins very quickly, almost like the Gravitron at the fair.
What is a centrifuge?
You all lowkey treat this DNALC educator like they're a celebrity.
Who is Jeff?
This is the shift in temperatures used for heat shock:
_____ ---> _______ ---> ________
What is:
0 celsius ---> 42 celsius ---> 0 celsius
BamHI cuts the pAMP at this basepair.
What is 1120?
These are the two plasmids we manipulated in order to make pLIG.
This device condenses electricity from the outlet in the table so the voltage can be increased to upwards of 150 volts.
What is the electrophoresis power supply, or power supply?
This is the name of the mummy in the museum exhibit.
Who is Otzi?
This is the information you would need in order to calculate transformation efficiency.
What is the number of colonies per microgram of plasmid DNA spread on the plate?
(# of colonies, mass of DNA, Fraction of cell solution spread on the plate)
HindIII cuts pKAN at this basepair.
What is 233?
This is the total base pair length of pAMP.
What is 4539 bp?
This is the name of the type of food we like to grow bacteria on. It originally comes from a seaweed.
What is Agar?
This is the year that Cold Spring Harbor Laboratory first opened. (I will accept within 5 years)
What is 1890?
In order for bacteria to survive on an AMP/KAN plate, it would need to have been transformed with any of these combinations of plasmids.
What is the simple recombinant (pAMP/KAN), the super plasmid, or a double transformation with pAMP and pKAN.
This is what you call the extra lengths of DNA that hang off (single stranded) after the restriction enzyme makes it's cut.
What are sticky ends?
This is the total base pair length of the simple recombinant plasmid (pAMP/KAN)
What is 5616 bp?
These tubes have a loose lid to allow for bacteria to aerate when making overnight cell cultures.
What is a snap cap tube?
This is the name of the Nobel Prize Laureate that helped discover the structure of DNA in 1953. He still lives at Cold Spring Harbor Laboratory today.
Who is James Watson?