(Blank)
What is a vector?
A plasmid is a natural example of this used in gene cloning.
WHAT IS PLASMID?
ITS A SELF REPLICATING,CIRCULAR,EXTRACHROMOSOMAL DNA & USED AS AVECTOR
The method of separation and isolation of DNA fragments
GEL ELECTROPHORESIS
What are restriction enzymes?
These enzymes produce sticky ends
Name the restriction enzyme that recognizes this palindrome GTCGAC
EcoRI
What is a primer?
This consists of a sequence of 15-25 bases and is crucial in the annealing step
What is a selectable marker?
An antibiotic resistance gene in your vector is an example of this.
What is chemical production to kill insects?
The genes of Bt corn allow for the expression of genes related to this.
DNA fragments join at
sticky ends
What is reverse transcriptase?
This enzyme creates DNA from RNA.
What is Agrobacterium tumefaciens?
This bacteria produces crown galls and is used to genetically modify plants.
What is reverse transcriptase PCR (RT-PCR)?
This type of PCR starts with RNA to generate cDNA
what is the enzyme used for linking of antibiotic resistance gene?
DNA Ligase
What is DNA sequencing?
A restriction map is generated from cutting plasmids with restriction enzymes and this other method
Name the two types of restriction enzymes.
Exonuclease and Endonuclease