Vocabulary
Small circular piece of DNA found inside a bacteria cell
Plasmid
Gel electrophoresis utilizes an electric current to cause fragments of DNA to move down the gel. DNA would move towards this end of the plate (positive or negative) and this is why? **Double Jeopardy Question**
What is the positive end AND because DNA is negatively charged.
What types of cell(s) are needed for cloning?
Egg cell and a body cell
Section of the genome that differentiates between plasmid and desired gene **Double Jeopardy**
Genetic marker
Function of Gel electrophoresis
Separate DNA fragments
Term used to refer to an organism that contains genes from other organisms
Transgenic
This is the name of the enzyme that cuts DNA at specific recognition sequences.
What is a restriction enzyme?
This is what happens is the electricity is left on too long during gel electrophoresis.
What is all of the fragments will fall off the end?
This is the name of the small circular piece of DNA that some bacteria contain.
What is a plasmid?
Golden retrievers are an example of this type of selective breeding
Inbreeding
Continued breeding of organisms with similar traits **Double Jeopardy Question**
Inbreeding
The DNA moves through the gel based on size. Identify where larger bands would be and smaller bands would be.
Larger bands would stay near the top and smaller bands would move toward the bottom
DNA produced by combining DNA from different sources
Recombinant DNA
What is the ultimate source of genetic variability?
MUTATION
The crossing of a lion and a tiger to produce a liger is an example of
Hybridization
This is the difference between a DNA fingerprint and a regular fingerprint.
What is a DNA fingerprint is a pattern of bands on a gel made from fragments of DNA and a regular fingerprint is the pattern of skin ridges on the surface of an individual's skin?
I want to cut between the sequence GATTA in a long sequence of DNA. What do i use?
Restriction enzyme
How do scientists create oil eating bacteria?
Inducing mutations
I want a dog who is family friendly but also hypoallergenic because I’m allergic to dogs. What is this process called?
Selective Breeding
Looking at the DNA fingerprint on the board, can you conclude that Sam and Ted are biological sons of Sandy and Bob? What else can you tell about Sam and Ted by comparing their DNA banding pattern?
They are both adopted but they are identical twins.
Name the famous sheep who was cloned?
Dolly
What happens during transformation?
-desired gene is isolated
-desired gene is spliced into plasmid creating recombinant DNA
- plasmid is placed back inside bacteria
-Bacteria is placed inside original cell and new gene becomes part of genome
This is a definition for genetic engineering AND TWO ways that genetic engineering has been used to benefit society.
What is the manipulation of an organisms genome by adding or removing genes? Lots of answers.