PCR
The components needed for PCR
What are Taq polymerase, FP, RP, water, and DNA?
The effect of a missense mutation
What is coding for a different Amino Acid?
The reason that bacterial cells are treated with aggressive salts and heat shocked
What is increasing transformation efficiency?
True/False: Reverse transcription is a part of the central dogma (i.e. occurs inside the cell?)
What is false?
The number of Liters in mL and microL
What are 10^-3L and 10^-6L?
A database used to identify the intron and exon structures of a gene
What is BLAST?
Transposable elements
What are mobile segments of DNA with terminal inverted repeats?
The selective marker used in Experiment 3
What is the antibiotic Ampicilin?
The enzyme and template used for making cDNA
What are Reverse Transcriptase and mRNA?
The starting amount, in L, of water and 10x TBE required to make 5L of 1x TBE
What are 0.5L TBE and 4.5L water?
The steps of PCR
What are denaturation, annealing, and extension?
True/False: Single Nucleotide Polymorphisms (SNPs) can be indels and indels can be SNPs
What is false?
The function(s) of a promoter
What is the regulation site of gene expression and binding site for RNA polymerase (to initiate transcription?
The process for making mRNA from pre-mRNA
What is splicing?
mL needed of 12.5M TAE buffer to make 1L of 1M TAE buffer
What is 80 mL?
The number of DNA strands that will be obtained after 9 cycles in PCR
What are 512 DNA strands?
The difference between a Transposable element and an indel
What is a Terminal Inverted Repeat (TIR)?
The type of gene regulation for the Ampicillin-resistance gene
What is constitutive expression?
The complementary tRNA anticodon to the mRNA sequence: 5' GAUAAC 3'
What is 3' CUAUUG 5' ?
The difference between cDNA and gDNA in a gel
What is cDNA is a smaller fragment and will move faster through the gel?
A type of polymorphism that can NOT be detected using PCR
What is a SNP?
The two genes present on the pGlo plasmid inserted into the bacteria in experiment 3
What are GFP and AmpR genes?
The number of introns in a certain gene with 3 exons
What are 2 introns?