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I will force you to love chemistry.
ECM-GF binding
ECM in disease
Wildcard
Structures
100
Something that reacts with an azide.
What is an alkyne?
100
A high Kd means what for GF sequestration?
Weaker GF sequestration
100
This ECM component is generally highly unregulated in fibrosis.
What is type I collagen?
100

Production of AGEs is caused by...

Non-enzymatic glycation of proteins by sugars.

100
Name of this structure
What is N-hydroxysuccinimide?
200
The reactive groups that participate in SPAAC.
What are azide and cyclo-alkyne? Bonus: Advantage of SPAAC over CuAAC?
200
Two aspects of heparan sulfate structure that can regulate GF binding.
What are sulfation location and density?
200
Mechanical consequence of decreased GAGs in osteoarthritis.
What is decreased compressive strength?
200
Mechanical consequence of glycation.
What is tissue stiffening? (via collagen cross linking)
200
Name of this structure
What is maleimide?
300
The full name of NHS and what it reacts with.
What is 1) N-hydroxysuccinimide and 2) amine?
300

Most growth factors do not bind this ECM component

What is collagen I?

300
Fibrillar collagens replace this ECM structure in liver fibrosis.
What is the basement membrane?
300
Types of reactive groups available on GAGs.
What are carboxyls and hydroxyls?
300
The reason why cells aren't migrating through a hydrogel made from these components.
What is it lacks a degradable sequence?
400
An advantage of a bioorthogonal reaction.
What is that it cannot react with native biomolecules?
400

One reason why a cell might prefer a sequestered GF vs. producing more GF itself.

Lots of reasons: needs GF now, presentation is better on a solid substrate, local higher concentration...

400

Biological consequence of decreased GAGs in osteoarthritis.

What is cell differentiation to fibroblastic phenotype, decreased compressive modulus, or joint pain?

400

One drawback to using a reductionist approach to engineer an ECM-mimetic matrix.

What is loss of synergistic sequences, lack of higher-order structures, etc.

400
The reason why you will not use PEG-NHS to append the adhesive peptide sequence KQAGDV to your scaffold (all amino acids in sequence required for activity).
What is that you could disrupt bioactivity by binding to Lys primary amine?
500
Two amino acids whose side chains can be reacted using EDC.
What are lysine (K; NH2) with aspartic acid (D; COOH) or glutamic acid (E; COOH).
500
One way that you could modify a GF to increase its sequestration in vivo.
What is appending a high-affinity binding sequence to the GF?
500
Process that is impeded by liver fibrosis.
What is diffusion?
500
Other than transcription, one way that MMP activity is regulated.
What is activation from pro-form or inhibition by TIMPs?
500
The name of this reaction: a) in the presence of UV + photoinitiator and b) in the absence of UV/initiator
What is: a) thiol-ene b) Michael addition