Electrophoresis and PCR

Gel Electrophoresis A

100

Dried powder made from seaweed used to make the electrophoresis gel

What is Seaweed Agarose

200

Technique used to sort DNA strands according to length

What is Gel Electrophoresis

300

The filter that sorts the DNA strands according to their length.

What is Gel

400

Causes the DNA to migrate through the gel

What is Electrical Current

500

The “unit” used to estimate the approximate lengths of the DNA strands in your sample

What is Base Pairs (bp)

Gel Electrophoresis B

100

It affects how fast the DNA strands move through the gel

What is the length of the DNA strands

200

Where you pour the melted agarose

What is Gel Mold

300

Used to make wells or holes into the agarose gel where you place the DNA sample

What is Gel Comb

400

Added to the electrophoresis box, it conducts the electric current from one end of the gel to the other

What is Buffer Solution

500

Contains DNA strands of known lengths and aids in the estimation of the length of the DNA strands in your sample

What is DNA Size Standard

Gel Electrophoresis C

100

Apparatus used to transfer DNA sample into the holes in the electrophoresis gel

What is Micropipette

200

Substance that makes the DNA sample thicker, contains a dye that makes the sample easier to see. It is added to the sample before it is loaded into the gel.

What is Loading buffer

300

This solution contains Ethidium bromide, which binds up to DNA and shows up under fluorescent light

What is DNA Staining Solution

400

Used to see the stained DNA bands

What is UV Light Box

500

Large groups of DNA strands that are grouped together in the gel

What is Bands

PCR Facts

100

What does PCR stand for?

What is polymerase chain reaction?

200

What can PCR be used for?

What is to multiply a strand of DNA millions of times?

300

What is a short strand of DNA used to target a region of DNA for replication by PCR?

What is a primer?

400

True or false: PCR is best used with DNA strands that are no longerthan a couple hundred bases?

What is true?

500

How much DNA is required for PCR

What is one billionth of a gram

PCR Steps

100

What temperature do the DNA strands have to be heated to?

What is 94 degrees celcius?

200

What happens when the DNA strands are heated?

What is they separate or "unzip"

300

What is the end result of PCR?

What is two complete pairs of double stranded DNA

400

True or False: The PCR cycle is only done once on a DNA segment.

What is false?

500

How long does each PCR cycle take?

What is less than 2 minutes?