Instruments and Chemistry
These are the three instruments sold by Element.
1)AVITI LT
2)AVITI
3)AVITI24
This is the difference between sequencing third-party libraries using Freestyle versus third-party libraries using Cloudbreak.
Cloudbreak requires on-the-bench (Adept) conversion. Freestyle does not.
These are two differences between Elevate libraries and third-party libraries.
-Adapter sequences (Elevate uses SP5 and SP27)
-Different sequencing primers
-Elevate Index 1 includes three random nucleotides at the begining for use in polony map generation
-Elevate sequences only on AVITI
This software allows customers to remotely monitor sequencing and cytoprofiling runs in real-time, visualize metrics to check run quality, and perform basic troubleshooting.
ElemBio Cloud
These are the analytes the AVITI24 is capable of analyzing for customers today.
1)RNA
2)Protein
3)Cell Morphology
The acronym ABC stands for this.
Avidite Base Chemistry
These sequencing kits have a Q-score spec of >70% Q50.
UltraQ
Element offers these two Elevate library prep kits.
1)Mechanical Shearing
2)Enzymatic Shearing
Where are fastq files generated from sequencing runs and what software is used to generate fastq files.
FASTQ files are generated off instrument using Bases2Fastq.
The AVITI24 has up to this much higher output than the standard AVITI.
50%
These are three differences between the AVITI LT and the AVITI.
AVITI LT is not compatible with these AVITI capabilities:
-UltraQ
-Trinity
-HD Mode
-High Output Sequencing Kits
-Advanced Run Settings
This Cloudbreak Freestyle kit size is not available as a Cloudbreak kit.
Freestyle 2x75 Low Output
These companies offer Elevate library prep solutions.
1)IDT
2)Twist Bioscience
This software is used for reprocessing cytoprofiling runs.
Cells2Stats
These are the well formats currently available for Teton flow cells.
1)Single well
2)12 well
3)48 well
The AVITI24 has all the capabilities of the AVITI. Additionally, it can run these assays.
Teton (Cytoprofiling)
This is the total number of cycles available in a 2x150 sequencing kit
334
These are the validated workflows for Trinity.
1)xGen Exome
2)Twist for Element 2.0 + Comprehensive Spike-in Standard Hybridzation
3)Twist for Element 2.0 + Comprehensive Spike-in Fast Hybridization
Name four supported data storage locations.
1)ElemBio Catalyst
2)AWS
3)GCS
4)DNANexus
5)Local Server
6)USB Drive
Cytoprofiling data is in an open format. List two third-party software programs users may use to analyze their data.
1)Seurat
2)CellProfiler
3)Python (Scanpy)
4)Cellpose
In ABC chemistry, ________ are used for detecting the base and ______ ________ are used for extending the sequence.
Avidites, Blocked Nucleotides
These are the sequencing primers available in the Adept primer vials and the sequencing primers available in the Freestyle kits.
Cloudbreak: TruSeq and Nextera
Freestyle: Elevate, TruSeq, Nextera, smallRNA
These two product lines are only compatible with Elevate libraries.
1)UltraQ
2)Trinity
These are the premium secondary analysis workflows available with ElemBio Catalyst.
1)Sentieon Germline DNA WGS
2)Sentieon Germline DNA Enrichment
3)NVIDIA Parabricks Germline DNA WGS
4)NVIDIA Parabricks Germline DNA Enrichment
This Element software program for visualizing cytoprofiling runs requires customers to first reprocess their cytoprofiling data using this other Element software to generate the necessary visualization files.
CytoCanvas requires reprocessing with Cells2Stats to generate the necessary visualization files.