Show:
Husbandry
Terminology
Regulations
Guest Speaker
Lab Settings
100
Can cedar wood chips be used as rat bedding? Name a possible consequence that can result from the use of this.
Cedar wood chips should never be used in research. They can cause harmful physiologic results.
100
What does IACUC stand for?
IACUC stands for - Institutional Animal Care and Use Committee.
100
***Daily Double*** What was the first law passed in 1966 that protected nonfarm animals in the United States?
Laboratory Welfare Act (also now known as Animal Welfare Act)
100
Uses the guide for the Care and Use of Laboratory Animals as it’s primary reference for evaluating laboratory animal care and use programs?
AAALAC ( Association for Assessment and Accreditation of Laboratory Animal Care)
100
Name 3 ways that individual mice can be identified in a cage of mice?
1. Ear punch 2. Ear tag 3. Ear or tail tattoo 4. SQ microchip 5. Temporary fur dye
200
***Daily Double*** How often is it recommended that mice be fed?
Ad libitum ( free Feed )
200
Define Sentinel
Cage at the bottom of the rack that gets a little bedding from each cage in the colony, then one animal is euthanized to test overall group health.
200
What was the original purpose of the Animal Welfare Act?
To protect owners of dogs and cats from theft of their pets and prevent sale of or use of dogs and cats that have been stolen. As well as ensure that humane care and treatment are provided to animals in research facilities.
200
List 5 USDA requirements for animals protocols?
1.# animals used 2. activities being preformed 3. proposed euthanasia 4. Species and why selected 5. Alternatives to painful procedures.
200
What form of protection does OSHA require animal caretakers to have access to in rooms that house pigs?
Ear protection.
300
What two vitamins are NHP not capable of synthesizing?
Vitamins C and D2
300
What does AWA stand for, and what update happened in 1990?
AWA - Animal Welfare Act, in 1990 there was an amendment requiring enrichment as a part of lab animal life.
300
***Daily Double*** Which species are not covered by the Animal Welfare Act?
1) Birds 2) Rats of the genus rattus 3) Mice of the genus mus 4) Horses not intended for research use 5) Other farm animals intended for use in improving animal nutrition, breeding, management, food and fiber.
300
What are the three Rs in animal research.
1. Reduction - reducing the number of live animals used in research 2. Refinement - using methods to minimize suffering and to improve the welfare of a lab animal 3. Replacement - replacing the use of live animals in research with the use of models, statistics, etc.
300
***Daily Double*** This room should be kept under negative air pressure relative to the corridor. A. Surgery Room B. Storage Room C. Rooms housing Nonhuman primates D. Rooms housing Amphibians/Non vertebrates E. Both C and D F. None of the above
C. Rooms Housing Nonhuman primates
400
What are the three main housing environments available in a research lab setting and give brief description of each?
Conventional, Barrier, Containment
400
What is the scientific name for a rat and where does it originate from?
Rattus norvigicus, it is from asia.
400
Once every ____, IACUC is required to inspect all animal facilities and study areas to review research facilities programs to ensure compliance with regulations and standards.
Every 6 months.
400
OLAW requires how many members on their team? What what their title include upon hiring?
6 members. 1. Vet 2. Animal Researcher 3. Nonaffiliated (unbiased) 4. Non-Scientist 5. Safety Officer 6. X (Anyone)
400
Most dry animal diets that are properly stored can be used up to ___ after milling.
6 months
500
At what percent of humidity must a rat’s environment be to prevent ringtail?
50%
500
What is phenotype vs genotype and what is an example of each?
1. Phenotype - what you can see and how a trait is being expressed (i.e. visible, behavioral, demonstrable, inducible, physiological) 2. Genotype - Genetic information specific to an individual, gotten by retrieving genetic info i.e. cheek swab, hair sample, tip of tail, chunk of flesh.
500
List four standards the IACUC must follow?
1. Reviews animal use protocols and monitors ongoing activities 2. Assures compliance of the animal care program and facilities 3. Investigates animal welfare concerns 4. Reports directly to the I.O.
500
A person can develop allergies from rodents when working in a laboratory setting with these species, this exposure is due to the ____ in the _____. What methods can reduce this exposure?
Protein; Urine. Proper PPE and ventilation.
500
Give 4 examples that would help reduce the facility's containment control.
1. Laboratory design could include entry barriers 2. Designed traffic flow 3. Specialized ventilation housing racks 4. Biological safety cabinets 5. Extensive personal protective equipment.