What is a piece of exogenous self replicating DNA introduced and expressed by bacteria independent of the bacterial DNA
100
What is the central dogma of biology?
What is replication of DNA --> transcription into RNA --> translation into protein
100
What makes up the primary structure of a protein?
What is the list, in order, of amino acids
200
The feature that defines a Gram+ bacteria is...
What is thick peptidoglycan layer
200
What plasmid will we be using and what protein will be expressed that allow us to visualize if the bacteria have been transformed
What is pGLO and GFP (green fluorescent protein)
200
Replication of the bacterial plasmid will begin at...
What is the origin of replication
200
You have a stock solution that is 10mg/mL. You need to make a 6 mL of a solution that is 3.5mg/mL. How much of the stock and solvent would you need in uL?
What is 2100 uL of stock and 3900 uL of solvent.
300
Describe what occurs to the bacteria and nutrients during the stationary phase
What is bacteria are no longer dividing or are dividing and dying off in equal numbers; nutrients are becoming scarce
300
Generally speaking, what percentage of cells take up the plasmid and are tranformed?
What is 1-10%
300
Where did GFP come from?
What is bio luminescent jelly fish
300
What is the Science and Media assignment? When is it due?
What is you must find a popular news article about a scientific study or idea; then find a peer reviewed journal article that also discusses this and compare the two. It is due the day of the final exam (11:59PM through blackboard)
400
A bacteria does not require oxygen for cellular respiration, is rod shaped and has a thin peptidoglycan layer. How would you describe this bacteria?
What is Gram -, aerobic and bacillus
400
What selection marker will our plasmid contain?
What is bla (ampicillin resistant expressed on beta lactamase gene)
400
How is antibiotic resistance conferred?
What is
antibiotics select for bacteria that can live in the presence of that antibiotic, these genes can then be passed onto other bacteria
400
Why can you not use a Michaelis-Menten plot to determine the Vmax of an enzyme?
What is because Vmax is approached, but never reach on a MM plot
500
Give an example of a spirillus bacteria
What is the bacteria that causes lyme disease or syphilis (Borrelia or Treponemapallidum)
500
Give the 4 requirements that a plasmid must have in order to work
What is
1. origin of replication
2. Marker or way to select for transformed cells
3. Restriction sites for cutting
4. With 2 genes of interest, must have different origins
500
In the plasmid, arabinose C will control the transcription of which gene(s)
What is GFP, araA, araB, araD
500
When genes are unlinked, their percent recombination will be...