Agar
The color of a positive MR tube
What is bright/brick red?
These are byproducts of the urease enzyme in the presence of urea
What are CO2 and ammonia?
This organism is indole and lactose positive, TSIA A/Agas, and has a green metallic sheen on EMB
What is Escherichia coli?
These are hairlike structures that assist in adherence to host cell surfaces
What are fimbriae or pili?
Enterobacterales are classified with this type of oxygen requirement
What are facultative anaerobes?
The pH indicator in an amino acid decarboxylase tube
What is bromocresol purple?
Tryptophan can be hydrolyzed into this helpful differentiating biochemical product and will turn baby blue when mixed with DMACA
What is indole?
This organism is urease and PAD positive, lactose negative, TSIA K/AH2S, and swarms on BAP
What is Proteus?
This structure is only found on motile organisms, is heat labile, and is used to serotype Salmonella
What is H antigen (flagellar antigen)?
This serotype of E. coli can cause bleeding of the intestine, hemolytic uremic syndrome, and has a subgroup that can be identified with the Shiga-toxin rapid test
What is Enterohemorrhagic Escherichia coli (EHEC)?
The result of a SIM tube that shows growth radiating from the innoculating stab and a red ring following the addition of Kovac's reagent
What is - + +?
This tests for lactose fermentation via the beta-galactosidase enzyme and will turn yellow when positive
What is the ONPG test?
This organism is DNase and ONPG positive, lactose negative, TSIA K/A, and has brick red colonies on BAP
This structure is part of LPS, heat stable, and can be used to serotype Shigella and Salmonella
What is O anitgen (somatic antigen)?
This species of Shigella is the most common, serogroup D, and is ONPG positive
What is Shigella sonnei?
The selective and differential agents in MAC agar and the organisms they are selective against
What are bile salts, crystal violet, and neutral red - gram pos and fastidious gram neg?
This TSIA result indicates that only glucose has been utilized for fermentation
What is K/A?
This organism is lactose and ONPG negative, TSIA K/AH2S, and will have black colonies on HE
What is Salmonella?
This structure derives its toxicity from Lipid A in LPS and can cause fever, hypotension, and DIC
What is endotoxin?
The small inoculating loop is calibrated to deliver this amount of liquid
What is one microliter?
This agar screens for Salmonella and Shigella and will turn bright red when lactose is fermented
What is XLD?
This fermentation pathway is identified with the VP test
What is glucose -> acetoin -> 2,3-butanediol?
This organism is citrate and ONPG positive, lactose positive or negative, and TSIA can be A/A or K/A, with or without H2S production
What is Citrobacter?
This structure is heat labile, covers the O antigen, and is found in Klebsiella, Salmonella, and E. coli
What is K antigen (envelope antigen)?
What is inoculum control (positive control)?