Procedure
What fruits were used?
What does the apple interact with that causes it to brown?
Oxygen.
What is the function of an enzyme?
To catalyze or speed up chemical reactions.
What is an anti-oxidant?
a substance that inhibits oxidation, used in preservatives
What are all of our names?
Emily, Taylor, Stephanie, Makayla
How do you prevent cross-contamination?
Cut the apple first, then the lemon.
What does PPO stand for?
Polyphenol oxidase
What is the active site?
Where the substrate binds to the enzyme.
Define "denature"
the action of inactivating an enzyme by altering its structure and bonds due to an unfavorable temperature or pH
How many of us have have siblings?
All of us
How often should one check the experiment?
Every 10 minutes for an hour.
Is lemon juice acidic or basic?
Acidic.
What is the induced-fit model?
The enzyme shape alters to correctly bind to substrate.
What is pH?
the measure of a hydrogen ion concentration of a solution, effects enzyme activity
What grade are we in?
11-junior year
What's the difference between the experimental and control group?
The experimental group receives the treatment, whereas the control group does not.
3
How do enzymes impact activation energy?
Lowers it.
Define melanin
a dark brown to black pigment occurring in plants and animals
Who is the advisor of SNHS?
Ms. Gomez
What is an independent variable?
The factor that is manipulated (lemon juice application)
Explain: What is enzymatic browning?
The conversion from phenolic tissue to melanin.
What two factors impact enzymatic activity?
Temperature and pH.
Define polyphenol oxidase
enzyme that contains copper, catalyzes the oxidation of the phenolic compounds found in plant tissue
What kind of coat can only be put on when it is wet?
A coat of paint