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General Enzyme Knowledge
Activation Energy
Enzymes
Enzyme-Substrate Complex
Enzyme Lab
100
Enzymes are named after the substrate they react with - replacing the "ose" with "ase". This is the enzyme that breaks down Lactose.
What is Lactase?
100
The energy needed to start a reaction is called this.
What is activation energy?
100
Enzymes _________ reactions in the cells.
What is speed up?
100

This is the term used when the enzyme is actively operating on the substrate causing the reaction.

What is an enzyme-substrate complex?

100

True or False, Hydrogen peroxide was the substrate in this lab.

What is True.

200
True or False: Enzymes are used up during their reaction.
What is False? Enzymes do not get used up. They are free to react again and again. This is why they are only need in the body in small amounts.
200
True or False: All reactions require the same amount of ENERGY to get started.
What is false?
200
Enzymes are this type of macromolecule.
What is proteins?
200
This is what the Enzyme acts upon.
What is a substrate?
200

True or False, Catalase was the enzyme in this lab.

What is True.

300
Explain what is meant by "Enzymes are substrate specific"
What is they only react with certain substrates that fit into the active site. Think Lock and Key!
300
This protein will reduce the activation energy.
What is an enzyme?
300
Enzymes lowers activation energy & speeds something up. Another word for this is a ____________.
What is catalyst?
300
Where the specific substrate fits in the enzyme.
What is an active site?
300

This is where the enzyme came from for this lab.

What is the liver?

400
Describe the Lock and Key hypothesis using the terms substrate and enzyme. Which is the lock, which is the key?
What is Enzymes only react with certain substrates that match their shape correctly. Enzymes are the lock, substrates are the key.
400
An enzyme will ____________ the amount of activation energy required.
What is reduce?
400

The type of enzyme inhibition in which the inhibitor binds to an alternate location on the enzyme changing the shape of the active site.

What is allosteric (non-competitive) inhibition?

400
The “new” substance created during reaction.
What is a product?
400

This was the type of reaction we witnessed in the Catalase lab. (synthesis or hydrolysis)

What is Hydrolysis

500
Describe the differences between a synthesis reaction and a hydrolysis reaction.
What is: synthesis is a building up reaction (2 substrates join into one) hydrolysis is a breaking down reaction involving water (1 substrate breaks into two)
500
Activation energy is the energy needed to start a ________.
What is reaction?
500
True or false: For each reaction, there is a specific enzyme.
What is true?
500

The most commonly accepted model for enzyme action, in which the enzyme conforms a bit to the substrate is called the _________.

What is the induced fit model?

500

This is why no bubbling was observed with the high temperature (boiled) liver.

What is the enzyme catalase was denatured due to the heat. The active site was no longer the same shape and could not react with the substrate hydrogen peroxide.