Enzymes
What is the shape of an Enzyme?
Globular
What is the definition for metabolism?
The sum of all chemical reactions in the body
What biomolecule does enzymes belong to?
Proteins
Example of an enzyme
Amylase, trypsin, pepsin, maltase, lactase, lipase, DNA polymerase
What does optimum mean?
The level the enzyme works best at
Optimum pH for catalase in the liver
pH 7
Optimum temperature for catalase in the liver
37oC
Why is washing up liquid used in enzyme experiments?
To create bubbles; to measure the volume of froth/foam produced; measure rate of reaction
Optimum temperature for catalase in celery
25oC
Optimum pH for catalase in celery
pH 9
What is a Catabolic reaction?
The breakdown of complex (larger) molecules into smaller molecules
What happens when an enzyme losses its shape?
It losses its function
An application of bioprocesses
Antibiotics, sweeteners, sweet tasting frucose in soft drinks etc....
What is an Anabolic reaction?
The build up of smaller molecules into complex molecules
What is an immobilised enzyme
Enzymes trapped in an inert gel material
The enzyme pepsin works in the stomach..... at what pH?
optimum pH of 2
What is denaturation?
The enzyme losses its shape and function
What temperature does enzymes denature at?
60oC
what is a bioreactor
A vessel in which biological reactions occur
Hydrogen peroxide
H2O2
What is an Immobilised enzyme?
Enzymes that are fixed/trapped in an inert gel material.
Enzymes can’t move.
What is a bioreactor?
A vessel in which biological reactions occur
A substance that speeds up a reaction without being used up in the reaction itself
What is enzyme specificity?
An enzyme will act on one particular substrate: at the Active site
An enzyme and substrate perfectly binded together is known as
Enzyme-substrate complex