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100

This petite, brunette Jedi Master in the Weaver lab holds dominion over training and scheduling for the EV Center's ZetaView NTA.

Who is Cara Bekas?

100

This is the supernatant containing EVs and other secreted factors that we collect from cells in culture.

What is conditioned media?

100

 

Hardin, Heuser and Stahl first described release of exosomes from this endosome-derived organelle.

What is the multivesicular body?

100

Of this class of membrane-bending transmembrane proteins, CD63 is most highly enriched in exosomes.

What are tetraspanins?

100

These large EVs are released from cells undergoing programmed cell death.

What are apoptotic bodies?

200

This high tech wizard of lipidomics and RNA-seq is friend to two and four legged creatures alike!

Who is Kasey Vickers?

200

You have a need for speed if you want to separate large EVs, small EVs, exomeres and supermeres using this method.

What is differential centrifugation?

200

Exocyst-containing ectosomes are shown here budding from these microtubule-based antenna-like organelles.




What are cilia?

200

Hrs is a subunit of this machinery, which recognizes ubiquitinated proteins and targets them for incorporation into ILVs.

What is ESCRT (ESCRT-0)?

200

Fragments and even wholly intact examples of this energy-producing organelle have been revealed inside various types of large EVs by electron microscopy imaging.

What are mitochondria?

300

According to this pathology and immunology phenom, we wheeze and sneeze and … release EVs!

Who is Heather Pua?

300

This technology tracks the Brownian motion of individual particles to determine EV size and concentration.

What is Zetaview/nanoparticle tracking analysis?

300

You can take pictures like this of cargoes on individual EVs using this piece of EV Center equipment.

What is the ONI Nanoimager?

300

The term “exosome” was first co-opted by this pioneer of the EV field, who once described her scientific journey as “Alice in Blunderland”.

Who is Rose Johnstone?

300

These uncommonly large EVs are associated with a highly metastatic “amoeboid” tumor cell phenotype.

What are large oncosomes?

400

This EV Engineering Wizard and Time-Traveling Mad Scientist share a passion for metabolic engineering, biofuels and flux analysis! 

Who are Jamey Young and Doc Brown?

400

These super-light particles may still be floating in your supernatant after you isolate EVs from cell media.

What are supermeres?

400

 This electron micrograph depicts the cup-like shape of these endosomally-derived small EVs.

What are exosomes?

400

Publishing within a week of each other in 1983, two independent studies of this transport protein led to the discovery of exosomes.

What is the transferrin receptor?

400

Ectosome budding requires molecular rearrangements of the plasma membrane and this underlying structural network.

What is the actin cytoskeleton?

500

This high-tech Phenom is the Olympic gold medalist of Tweezing EVs !

Who is Justus Ndukaife?

500

EV fingerprinting uses this fluorescent fluidics analysis to classify EV subpopulations.

What is flow cytometry?

500

 These small but mighty nucleic acids can hitch a ride in EVs to alter  gene expression in far-off recipient cells

What are miRNA?

500

Rab27a is required at this step in exosome biogenesis involving the MVB and the plasma membrane. 

What is MVB docking?

500

This ESCRT protein is recruited to the plasma membrane by ARRDC1 to generate small microvesicles called ARMMs.

What is TSG101?