Microbes
This microbe grows at 25° C
S. mar
Total Magnification
Scanning ____x ____
Low power
High Power
Oil immersion
10x4 = 40
10x 10 = 100
10x 40 = 400
10x100= 1000
Saturated fats contain ____ bonds
single
Name the carbohydrate monomers
Fructose, glucose, and galactose
List the selective media and what they select for
MacConkey Agar: Gram - Enterics
Mannitol Salt Agar: Staphylococcus & streptococcus
Azide agar: Gram +
Eosin Methylene Blue Agar: G(-) Enterics
Sarbouraud: Fungi
pink from phenol red.
Negative is yellow
If you wanted to test whether your mystery microbe was gram +, which media would you grow it on?
Azide to test for gram +, Mannitol Salt Agar, MacConkey for g-
Hydrogen bonds occur when
one element in a bond has a higher electronegativity than the H.
What are the differences between RNA & DNA
RNA - almost always single, ribose, uracil
DNA - double stranded, deoxyribose, thymine
Name and describe differential medias
Blood Agar: Hemolysis
• Mannitol Salt Agar: Mannitol Fermentation
• MacConkey Agar: Lactose Fermentation
• Urea Broth: Urea Hydrolysis
• Eosin Methylene Blue Agar: Lactose
Fermentation
• Carbohydrate Broth: Fermentation - Acid and
Gas
The products of fermentation are
Acid and gas
The concentration of H+
The nitrogenous bases of DNA are held together by
Hydrogen bonds
The total magnification of oil immersion is
1000X
E. coli is gram ___ ___
- Rod
Describe the 4 levels of the structure of proteins
primary - sequence of AA
secondary - alpha helix and beta sheets
tertiary - the folding of the structure
quaternary - combination of other proteins
semi solid media is used to
demonstrate bacterial motility.
Non motile will have a line, motile will be cloudy all over.
Describe dehydration synthesis
removes one -H+ from one molecule and -OH- from another to combine the molecules and produce H2O
Beta hemolysis is
the complete digestion of blood around a colony.