A visible population of microorganisms originating from a single parent cell and growing on a solid medium
What is a bacterial colony
The total magnification of a specimen with ocular lens magnification 10x and objective magnification 40x.
What is 400x
The creator of gram-staining
What is Hans Christian Gram
A medium that selects for the growth of one group of bacteria and inhibits the other.
What is a selective medium
Toxic by-products of oxygen utilization
What are hydrogen peroxide and oxides
A liquid culture medium used for bacterial growth.
What is broth
How well two objects can be distinguished from one another on a microscope
What is resolution
The dyes used in gram-staining
What are crystal violet and safranin
Media that support the growth of all bacteria but help you differentiate between different groups according to the reaction the bacteria has on the surface of the medium
What is differential media
Extremophiles microorganisms that thrive in high salt concentrations
What are halophilic bacteria
A needle-like shape of a bacterial colony
What is punctiform
The method used to identify the morphology and grouping of bacteria without retrieving any further information
What is simple staining
Bacteria that grows on EMB plate with a green metallic sheen
What is a gram-negative bacterium and a strong sugar fermenter
Microorganisms that grow better in O2, however, these bacteria can also grow in the absence of O2 with a lower growth rate
What are facultative anaerobes
The method used to isolate a single colony and have a pure bacterial culture.
What is isolation streaking
Bacterial morphology that is circular with 4 circular cells forming a square.
What is a coccus with tetrad arrangement
The gram-stained color of a gram-positive bacterial cell wall and that of gram-negative bacterial cell wall
What are purple and pink
Which bacteria can grow on a PEA plate?
What are gram-positive bacteria
A microorganism that thrives in extremely hot environments—from 60 °C upwards. The optimal temperature for the existence of hyperthermophiles is often above 80 °C.
What are hyperthermophilic bacteria
This sector has the highest bacterial population which is known as the "parent population"
What is sector 1
The step which allows bacteria to bind to the slide and breaks down the enzymes that can interfere with simple staining
What is heat fixing
The most important indicators of the success during gram-staining
What are the positive and negative controls
Which medium can bacteria that are purple in a gram stain grow (EMB or PEA)?
What is PEA plate
Microorganisms that can tolerate high pressures. They do not necessarily require these high pressures as they can grow and reproduce in less extreme environments along with high-pressure environments.
What are barotolerant bacteria