Linked Genes
Genes A and B recombine 12% of the time.
Genes B and C recombine 14% of the time.
Genes A and C are ____ cM apart.
What is 26 cM?
F1 has A/b a/B genotype. Given that A and B are 14% recombinant, the probability that a test cross with the F1 will produce a phenotype AB or ab is __%
What is 14%?
This type of RNA forms several catalytically active components of ribosomes.
What is rRNA?
The ribosome reads mRNA in this direction
What is 5' --> 3'?
This enzyme is responsible for zipping open DNA during replication. Be sure to list its alternative name if prokaryotes if necessary.
What is helicase or DnaB in prokaryotes?
Two true-breeding parents have a phenotype AbC & aBc
The double crossover phenotypes from a test cross of this F1 are __.
What are ABC and abc?
A--25-B
Given the parental phenotypes are Ab and aB, the probability that an AB phenotype will be obtained is __%
What is 12.5%?
This type of RNA Polymerase transcribes the 3 ribosomal genes.
What is RNA Polymerase I?
This is the specific site which release factors bind to during translation.
What is the A site?
This enzyme is responsible for preventing supercoiling.
What is topoisomerase?
A--20cM--B--20cM--C
F1 Genotype: A/B/c a/b/C
The probability of an ABC individual is __%
What is 16%?
A--20cM--B--10cM--C
Observed DCOs: 1
The interference in this progeny is __.
What is 0.5?
This type of enzyme transcribes tRNA, snRNA, rRNA, miRNA, and siRNA
What is RNA Polymerase III
This is the difference between a 70s and 80s ribosome. (Indicate which is part of which type of organism)
What is bacterial (70s) and eukaryotic (80s)?
Label the following forms of replication (conservative, semiconservative, dispersive)
(Left to right)
What is conservative, semiconservative, & dispersive?
A--10cM--B--20cM--C
F1 Genotype: a/b/c A/B/C
Probability of ABc is __%
What is 18%?
Given A, B, C, and D are all on the same gene & the following map distances, label the order of genes & the map distances between them.
What is:
A--12--B--13--C--6--D?
These are the three types of mRNA processing.
What are:
- Poly-A Tail
- 5' G Cap
- Splicing out Introns
3' UCA 5'
This tRNA could wobble to code for these amino acids.
BONUS (DOUBLE POINTS): If it wobbled, what kind of mutation would occur?
5' AGU 3' Serine
5' GGU 3' Glycine
BONUS: Missense Mutation
This enzyme removes nucleotides of RNA primers & replaces them with DNA nucleotides. It also reads from 3' to 5'
What is DNA Polymerase I?
A--10cM--B--30cM--C
The probability of no recombination given this gene is __%.
What is 63%?
A--10cM--B--30cM--C
The probability of any SCO given this gene is __%.
What is 34%?
This is known as modifications to the nucleotide sequence (and the protein produced) of some mRNAs
What is RNA editing?
These are three of the 5 steps to translational initiation.
What are:
- Small ribosomal subunit binds near 5' end of mRNA
- Initatior tRNA binds to start codon @ P site
- IF proteins help control ribosome formation & binding of initiator tRNA
- GTP provides energy
- Large subunit joins small to form intact ribosome, release IF, and begin translation
These are at least 3 of the 5 things required by PCR.
What are:
- A double-stranded DNA template containing the target sequence to be amplified
- A supply of the four DNA nucleotides
- A heat-stable DNA polymerase
- Two different single stranded DNA primers
- A buffer solution