Lab Skills
UV Mutagenesis
Eukaryotic Cell
Glow Lab
Cell Cytoskeleton
100
Basic stain used for Exercise 9 (Eukaryotic Cell)
What is Iodine?
100
The intensity of UV light and time of treatment
What is dosage?
100
Information hub in cells
What is the nucleus?
100
The plasmid used in this exercise
What is pUC18?
100
Name of an organism with cilia or flagella that was observed in this exercise
What are Amoeba proteus, Stentor, or Euglena?
200
The genetic alteration of a cell resulting from uptake and genomic incorporation of environmental genetic material (DNA)
What is Transformation?
200
Binds to thymine dimers and is excited by visible light
What is photolyase?
200
Stain for mitochondria
What is Janus Green?
200
Inactivates ampicillin and other penicillins
What is B-lactamase?
200
The 3 components of the cytoskeleton
What are actin microfilaments, intermediate filaments, and microtubules?
300
The type of microscopy used to localize fluorescent molecules in a biological sample
What is fluorescence microscopy?
300
DNA replication is halted with thymine dimers because this enzyme can't load onto DNA
What is DNA Polymerase III?
300
3 structures found only/mostly in plant cells
What are Chloroplasts, Vacuoles, and Cell Wall?
300
3 organisms that have the luciferase enzyme
What are fireflies, sea pansies, and click beetles? (also: Vibrio fischeri, etc)
300
The functional unit in striated muscle tissue
What is the sarcomere?
400
The stain used in Exercise 11 to slow movement of organisms
What is Protoslo?
400
The controls in this experiment
What are the non UV-irradiated plates?
400
The 3 attributes that mitochondria and chloroplasts share that indicate they were once free-living bacteria (and later engulfed = endosymbiosis theory)
What are binary fission, own circular DNA, and own ribosomes?
400
The ability of a cell to take up extracellular DNA from its environment
What is competence?
400
The structure constructed during replication to enable separation of the chromatids
What is the mitotic spindle?
500
The organism used in Exercise 8 (UV Mutagenesis)
What is Serratia marcescens?
500
The four types of DNA damage repair mechanisms
What is Photoreactivation, Excision repair, Recombination repair, and SOS-repair?
500
The movement of cytosol and organelles within plant cells
What is cytoplasmic streaming?
500
The chemical used to make competent cells (and how) before the exercise
What is calcium chloride which permeabilizes cell walls?
500
The motif found for flagella and cilia
What is the 9+2 motif?