Biochemical Tests For Unknown
Community Profiling
Horizontal Transfer of Genetic Material
Hand-Washing
Miscellaneous
1

This test measures the presence of motility, indole, and sulfur production

What is SIMS?

1

This is the study of microbiome around us, in our communities (the subway, populated gardens, etc.)

What is Urban Metagenomics?

1

These small circular pieces of DNA can be transferred from other cells or from the environment

What are plasmids?

1

True or False: Microorganisms are ubiquitous

What is True?

1

GFP produces this color under UV light

What is green?

2

Got a negative result? Don't forget to add zinc to this test. Beware! Don't add too much!

What is the nitrate reductase test?

2

This region of bacterial DNA that helps make polypeptides is used for PCR when one wants to detect the total number of species in their sample

What is 16s rRNA?

2

We used this species of bacteria for transformation experiments

What is E. coli?

2

You came up with two of these for your experiment - an independent and dependent one

What are variables?

2

Ampicillin, penicillin, chloramphenicol, and tetracycline are types of these chemical compounds

What are antibiotics?

3

The end result of this test resonates with Barbie.

What is the Urease Test?

3

PCRs requires this enzyme that is used by bacteria that often live in geysers and deep-sea vents

What is Taq polymerase?

3
In pGLO, fluorescence is triggered by the presence of this sugar in our media

What is arabinose?

3

Soap has both polar and nonpolar properties, meaning it is this

What is amphipathic?

3

The region around an antibiotic disc shows no growth. Make sure to use the metric system when measuring this circle!

What is the zone of inhibition?

4

Named after two scientists, this test detects the ability of a species to catabolize glucose

What is the Vogues-Proskauer Test?

4

Annealing *generally* occurs at this temperature

What is 55C?

4

This solution helps make our cells more competent

What is CaCl2?

4

Micrococcus luteus, Staphylococcus epidermidis, Staphylococcus aureus, Corynebacterium and Propionibacterium acnes make up the microbiome of this region of the body

What is the skin?

4

The arrangement and morphology of this bacteria is linear and spherical, respectively. Hopefully your throat doesn't hurt!

What is streptococcus?

5

Skim milk agar is used to detect organisms that break down this protein

What is casein?

5

These "building blocks" bind to the silica matrix when a high-salt buffer is introduced into the Spin-Column

What is DNA?

5

This region on a plasmid is valuable for when one wants to perform gene editing using restriction sites

What is the multiple cloning site?

5

This Hungarian physician claimed hand-washing would save lives! What an insane idea!

Who is Ignaz Semmelweis?

5

Using our soil sample, we attempted to isolate an antibiotic producer named this

What is Streptomyces?