NOBODY CALLS ME YELLOW!
It's more ideal to fix a specimen in Bouin solution rather than "Boone's solution" when you know GI or endocrine tissues are being prepped for this stain.
What is Trichrome?
This slightly-alkaline fixative is excellent for trephine biopsies of hematopoietic and lymphoreticular tissue, but mind your pH's and Qs: it has a strong potential for formalin pigment.
What is B-5?
Excessively bloody specimen? Better think hard before putting it in this additive noncoagulant fixative.
What is formalin?
16.6°C
What is the freezing point of glacial acetic acid?
One person in particular would like to see this aqueous non-coagulant additive move from permanent press to permanent fixture in fixation as a replacement for NBF.
What is glyoxal?
While not a typical ingredient in traditional Hollandaise sauce, this chemical compound reduces the RBC-lysing action of acetic acid to prepare specimens for special stain.
What is copper acetate?
This extremely-toxic stable fixative dissolves iron, but yields some excellent nuclear detail and even can be used as a decalcifying agent for bone marrow needle biopsies.
What is Zenker's fixative?
One slightly-acidic compound fixative will highlight specific granules in the adrenal medulla, but you might end up with this particular artifact from one rather toxic ingredient.
What is chrome pigment?
These specific types of alcohol can claim coagulant, non-additive status that preserve pigments and preserve water-soluble tissue components. Stay out of the medicine cabinet.
What is methanol and ethanol?
Davidson's fixative, used for these two whole organ specimens, is often a substitute for Bouin.
What are eyes and testes?
Like a fine wine, this fixative that preserves glycogen and other carbohydrates only gets better with age because ironically, it also contains this important ingredient which makes it memorable and functional.
What is Gendre's fixative and 95% ethanol?
While not a C2G consideration, this fixative commonly used in electron microscopy is provided in small ampules, must be used immediately upon opening and will fix ocular and nasal tissue upon contact.
What is osmium tetroxide?
When using Davidson's solution, you'll want to ensure that your two favored organs to be fixed are sufficiently ligatured lest you run the risk of this pigment.
What is formalin pigment?
Unlike formaldehyde, this substance renders lipids insoluable. It also preserves mitochondria and leaves the tissue much softer than expected after fixation. Keep your records straight with this one.
What is potassium dichromate?
You can find yourself carbohydrate-loading at a carnival with this non-additive coagulant that can also switch out one type of alcohol for another.
What is Carnoy's solution?
This buffered compound additive is no easy ride around the ice rink; it's most useful for this type of microscopy.
What is Zamboni solution and electron microscopy?
Helly's fixative highlights the intercalated discs of cardiac muscle and preserves red blood cells, but combines these two highly-toxic substances.
What is mercuric chloride and potassium dichromate?
Thorough rinsing is absolutely necessary when transferring a specimen from Hollande into a phosphate-buffered solution to prevent this atypical non-pigment artifact that cannot be remedied.
What is insoluble phosphate precipitate?
What is acetic acid and red blood cell lysis?
History is alive in this coagulant fixative that still finds occasional use despite its two simple non-aqueous ingredients.
What is Clarke's solution?
The fundamental reason one would be called "Yellow" is not political here, but due to this acid that causes extreme tissue shrinkage and does this to nucleic acids.
What is picric acid and hydrolysis?
This additive coagulant that prevents freezing and requires documentation at every step must only be used in compound fixatives.
What is mercuric chloride?
If you're lucky, this compound fixative will give you all the colors of the fixation rainbow
What is Helly's fixative?
The hardening effect of formaldehyde that nicely preserves spatial relationships in a section of tissue is achieved through this basic chemical process.
What is protein cross-linking and formation of methylene bridges?
While the phrase "pickling one's liver" might bring a different substance to mind, this noncoagulant protein cross-linking fixative from the same carbonyl group will preserve ultrastructures well.
What is glutaraldehyde?