Fixatives Part 2

What am I?

100

10% aqueous formalin is made up of 100 mL of 37-40% formaldehyde and this

900 mL of distilled water

200

37-40% formaldehyde, ethyl alcohol and distilled water

Alcoholic formalin

300

Mercuric chloride, sodium acetate and distilled water are my stock solution. When mixed with formaldehyde I become a working solution

B-5 

400

Mercuric chloride, potassium dichromate, sodium sulfate and distilled water are the stock solution for these

Zenker and Helly

500

Copper acetate, picric acid, formaldehyde 37-40%, acetic acid and distilled water make up this fixative solution

Hollande solution

What am I made of?

100

Bouin solution

Picric acid, 37-40 % formaldehyde, glacial acetic acid

200

Clarke solution is made of this

Absolute alcohol and glacial acetic acid

300

Zenker working solution

Stock- Mercuric chloride, potassium dichromate, sodium sulfate, distilled water

+ Glacial acid acid

400

Methacarn substitutes this for ethyl alcohol in Carnoy solution

Methyl alcohol

500

Gendre solution

95% alcohol saturated with picric acid, 37-40% formaldehyde, glacial acetic acid

Blast from the past

100

Oil red O requires this type of mounting media

Aqueous- organic solvents in resinous medias will dissolve the fat stained

200

Plasma cell cytoplasm is stained this color using the methyl green-pyronin technique

Red-rose

300

This hematoxylin solution must be used within a few days after being prepared

Weigert- ferric chloride will over oxidize due to being a strong oxidizer

400

In regressive staining hematoxylin is overstained and removed by this process

Differentiation

500

Treatment in a weak basic aqueous solution like ammonium hydroxide that changes the dye from red to blue is called this

Bluing

Problem Cases

100

The two major problems with fixation are delayed fixation and this

Incomplete fixation

200

Autolysis is caused when this happens

Fixation is delayed

300

Smudgy nuclei and nuclear bubbling can result when this occurs

Incomplete fixation

400

If the tissue is not well fixed when processing has begun, fixation continues in this causing the center to become more eosinophilic than periphery

Alcohol

500

Lugol iodine solution can remove this

Mercury pigments

Random

100

Unfixed tissue that needs to be held for several days or transported over a long distance should be placed in this

Michel transport medium

200

Formaldehyde, glutaraldehyde, and Zamboni solutions can be used for light microscopy and this

Electron microscopy

300

Specimens remain in fixative solutions are room temp indefinitely, penetrate quickly, and can fix tissues for light and electron microscopy are all advantages for this group of fixatives

Primary buffered PAF fixation (Zamboni solutions)

400

This is a nonaqueous fixative that is frequently used on frozen tissue sections for IHC

Acetone

500

The fixative solution is preferred for viewing chromaffin granules in the cytoplasm of the adrenal medulla

Orth solution