Forensic DNA Review

DNA Characteristics

100

What are are the four nucleotides of DNA?

What are Adenine, guanine, thymine, and cytosine?

200

This is where DNA is stored.

What is the nucleus?

300

Only __ % of the order of nitrogen bases in DNA is different.

What is 0.1%?

400

These are double ringed nitrogen bonds?

What are purines?

500

The noncoding segments of DNA?

Introns?

PCR

100

This step of PCR unwinds the DNA double helix.

What is denature?

200

This instrument is used for PCR.

What is a thermal cycler?

300

PCR stands for this. **DOUBLE POINTS**

What is polymerase chain reaction?

400

This is what PCR does.

What is amplification?

500

This is the PCR stage at which a low temperature is used to attach primers to the template strands.

What is the annealing stage?

CODIS

100

This is what CODIS stands for.

What is Combined DNA Index System?

200

The number of STR loci utilized by CODIS.

What is 13?

300

The year that CODIS was launched

What is 1998?

400

This is what CODIS keeps.

What is a national database of DNA profiles?

500

The amount of DNA needed for kits to genotype all 13 CODIS markers.

What is 1 nanogram?

DNA Markers

100

RFLPs stand for this. ***Triple POINTS***

What are Restriction Fragment Length Polymorphisms?

200

This what restriction enzymes do.

What is cut DNA at specific sites?

300

This is what STRs stands for.

What is Short Tandem Repeats?

400

What is a SNP?

What is single nucleotide polymorphism?

500

This is a location in the coding of the DNA that has variation.

What is polymorphism?

DNA Extractions & Electrophoresis

100

This is used to precipitate DNA.

What is alcohol?

200

This step is done to break the cell walls during DNA extraction.

What is lysis?

300

What is used to dissolve cell membranes?

What is detergent

400

This is used to remove proteins.

What is proteinase?

500

What direction does DNA move towards during electrophoresis?

What is towards the positive terminal?