Ligation
Transformation
Miniprepping
RE Digest
Sequencing
100
An enzyme that links two pieces of DNA together
What is DNA ligase?
100
The phase of bacterial growth when the nutrients in the growth medium are consumed
What is the stationary phase?
100
The name of the step where NaOH and SDS are add to the cells to cause them to burst
What is lysis?
100
An enzyme that cuts DNA at a specific site
What is a restriction enzyme?
100
The name of chain terminator sequencing method most widely used
What is Sanger Sequencing?
200
The part of a plasmid that is necessary to propogate the plasmid
What is the origin of replication?
200
The solid media on which bacteria grow
What is agar?
200
The name for the liquid above a pellet after centrifugation
What is supernatant?
200
All cut sites read the same forwards and backwards, which is know as this
What is a palindrome?
200
Nucleotides that cause DNA polymerase to terminate DNA synthesis
What are ddNTPs?
300
The antibiotic used to select for transformed bacteria
What is ampicillin?
300
After the DNA and bacteria are mixed together, this causes the DNA to pass through the bacterial cell wall
What is heat shock?
300
Where both genomic and plasmid DNA can be found in prokaryotic cells
What is the cytoplasm?
300
The type of cut that is made when there are overhangs left on the DNA strands
What is sticky?
300
The DNA sequence shown for the first 6 bases in the image
What is GAACTT?
400
The gene that, when expressed in the absence of an insert, is lethal to bacteria
What is EcoR47IR?
400
The name given to bacteria that are capable of taking up foreign pieces of DNA
What are competent cells?
400
A piece of DNA that results from being ligated into a plasmid
What is recombinant DNA?
400
The number of cuts made to a linear piece of DNA when four bands were visualized after electrophoresis
What is three?
400
The DNA sequence for the first 6 bases shown in the image.
What is ATGCCA?
500
The chemical used to activate the promoter of the lethal gene
What is IPTG?
500
The two chemicals used to make bacteria capable of taking up DNA
What are CaCl2 and DMSO?
500
The step when gDNA renatures and aggregates, and the high salt concentration precipitates the proteins
What is neutralization?
500
Cutting DNA into fragments and then figuring out their order
What is restriction enzyme mapping?
500
Terminator nucleotides are missing this functional group at the 3' position.
What is a hydroxyl group?