Spoilage
What is the difference between aerobic and anaerobic?
Aerobic = with air
Anaerobic = without air
How often are we supposed to clean our work benches?
before AND after
especially when switching projects & organisms
What does TVC stand for?
Total Viable Count
Countable range for Salmo
25-250
What is the importance of not contaminating a Surrogate Study?
These products get sent to food manufactures and get ran through their "process". Contaminating a food factory could cause harm and illness to the general public.
The PH of acidified MRS should be ____.
5.4 + or - 0.2
How many locations do we swab weekly for environmental testing?
10
What do we transfer Kraft Culture to?
Non-fat dried milk
What does VRBA stand for?
Violet Red Bile Agar
This organism is used as a surrogate for Salmonella
E. Faecium
10-150
What is the allowance given for sample prep?
5% above the target volume
What media is used to plate Kraft Culture heat shocked samples
RCA plates
Describe the difference between Typical and Atypical staph.
Typical colonies are circular, smooth, convex, moist, 2–3 mm in diameter, gray to jet-black, frequently with light colored (off white) margin, surrounded by opaque zone and frequently with an outer clear zone.
Atypical colonies may be less than jet black in appearance and/or be free of any opaque and clear zones.
When plating a liquid, how do you plate a 0 dilution when you have spread plates?
1 ml of the liquid (not buffered) across 3 spread plates.
This method is used to determine the Anaerobic Plate Count of raw meat
APC 3DRT
When pouring media, the temperature should be in this range.
45-48C
What two species of probiotic organisms is our internal total viable count method able to enumerate?
Lactobacillus and Bifidobacterium
Name 3 (there are 8) medias used in Salmonella AOAC qualitative method.
Acceptable Answers: Lactose broth, RV, TT, HE plates, XLD plates, BS plates, LIA Slants, TSI Slants
The time it takes at a given temperature for the microorganism population to go down 1 log is called _______.
D Value
Lactobacillus is _____.
A. gram negative, catalyse negative
B. gram positive, catalyse positive
C. gram negative, catalyse positive
D. gram positive, catalyse negative
D. gram positive, catalyse negative
When melting media in an autoclave or microwave what is the maximum temperature allowed?
105C
What is the concept of method validation?
Validating client methods in reference to known, approved methods.
Saving time and money **
Which is the TRUE Statement
A. All coliforms are E. Coli
B. All E. Coli are coliforms
B. All E. Coli are coliforms
What thermal death criteria validates a process for food production?
5-log reduction