What's Cookin'?
Martha Stewart's
Selection Recipe
Survival of the
Fittest
4 hours later...
Pump it
100

The carbon loading rate and pH setpoint for fermentations

What is 15 g/L and pH 6.0?

100

The selection recipe limits the presence of this element in the Selection Reactor (R-301)

What is Carbon?

100

The percentage decanted every 12-hrs from R-301's total working volume

What is 25%?

100

The production feed volume is what percent of the selection broth decant

What is 50%?

100

On a pump skid, this controls the PSI of material in recirculation.

What is the Flow Valve?

200

This carbon acidifies the reactor and prevents bacteria from doing their job

What is Lactic Acid?

200

The salt that provides phosphorus to the Selection Reactor (R-301)

What is potassium phosphate (KH2PO4)?

200

During this phase, PHA producers and others grow.

What is the feast phase?

200

PRs are more concentrated in ____ and less concentrated in ____.

What is carbon and nitrogen?

200

On the HMI, if this level is triggered a pump interlock will occur 

What is "high high alarm level"?

300

The preferred process flow for R-201 after anaerobic fermentation is complete.

What is R-201 to S-304 to TK-202?

300

The salt that provides ammonia to the Selection Reactor (R-301)

What is ammonium chloride (NH4Cl)?

300

This analysis tells how many grams per liter of cells are in the Selection Reactor (R-301)

What is cell concentration?

300

The Stabilization Tank (TK-303) stabilizes this property of PHA

What is the molecular weight?

300

This controls the system when operating in hand mode.

Who is the operator? 

400
This analytical instrument is used to track concentrations of specific carbon species. 

What is the HPLC?

400

The minimum working volume for the Selection Recipe Tank (TK-203)

What is 30%?

400
External controls that alter an organism's ability to survive.

What is selection pressure?

400

This is a supplemental material that is added to R-302 if only using a PM and AM decant from R-301

What is selection feed recipe?

400

These two items indicate flow on a pump skid and HMI.

What is the PIT and rotameter?

500

The 4 key intermediates produced in anaerobic fermentation that are metabolized into PHAs

What is Acetic Acid, Butyric Acid, Propionic Acid and Valeric Acid?

500

To shift the cell concentration in the Selection Reactor (R-301), this element would need to change in the recipe

What is the carbon concentration?

500

The cell residence time in the Selection Reactor (R-301)

What is 2 days?

500

The theoretical TC target for production feed

What is 6000 mg/L?

500

These two parameters can be used to control the air flow from the Blower (BL-505).

What is the scfm and DO?