Temp/Humidity
Husbandry
Sanitation
Euthanasia
H & I Protocols
100
What is the range that the relative humidity should be kept in an animal room?
30-70%
100
Name 2 IACUC violations
Overcrowded, Too Many Adults with Litter, Multiple Litters, Wean
100
What are the 3 processes used to either remove or kill microorganisms?
Sanitation, Disinfection, & Sterilization
100
Whose responsibility is it to ensure that the most humane standards are followed in performing euthanasia?
All personnel
100
What do "H" & "I" stand for? (Must have both correct)
Hazardous & Infectious
200
What is ringtail in a rat caused by?
Low Humidity
200
What is the microenvironment in an animal room?
The animal cage
200
What is sterilization?
The process that destroys all microorganisms on, and sometimes inside, an object.
200
Which publication is accepted by the research community as the standard for the euthanasia of animals?
The AVMA Guidelines for the Euthanasia of Animals
200
An Infectious protocol uses the "Biohazard Infectious Agent" sign. What color card is place on the infected cages?
Red
300
What is the acceptable temperature range for a mouse/rat room?
18-26 degrees Celsius or 64-79 degrees Fahrenheit
300
What kind of cages are designed to collect urine and/or feces?
Metabolic Cages
300
True or False: Animal holding facilities should be cleaned and disinfected according to a schedule set by a supervisor or facility manager.
True
300
What is reduced oxygen level in the brain and other tissues in the body called?
Hypoxia
300
What Animal Biosafety Level is the "Infectious Hallway" at BMSB?
ABSL2
400
According to the Guide, how many fresh air exchanges should an animal room have per hour?
10-15
400
In an animal facility with a clean/dirty corridor system, how many doors must each animal room have?
Two
400
When used properly, what do temperature strips indicate?
The highest temperature reached at the equipment surface
400
MS-222 is used for the euthanasia of what kind of animals?
Fish & Aquatic Amphibians
400
When can the signage (door & cards) be removed with a Hazardous protocol? With an Infectious protocol? (DOUBLE points if both are correct)
Hazardous: After hazard has passed. Infectious: After all infected animals have been euthanized.
500
Surgical suites and some animal rooms are maintained under (BLANK) pressure. Rooms designed to contain hazardous agents operate under (BLANK) pressure.
Positive, Negative
500
What is a HEPA filter? (What does HEPA stand for?)
High Efficiency Particulate Air filter
500
What does "contact time" refer to?
The period of time that a solution, such as disinfectant, is left on the surfaces until rinsed.
500
When verifying death in large animals, technicians may monitor the animal to verify the lack of a pulse, heartbeat, and breathing. In small animals, especially rodents, these vital signs are difficult to assess, making it necessary to use an adjunctive method to ensure that death has occurred. Name one of the 2 adjunctive methods discussed in training.
Cervical Dislocation & Open chest cavity with scissors or scalpel
500
Protocols using human cell lines are considered ABSL2, and require special handling. What suffix will the protocol number have (letter at the end)?
C