Running a Gel
PCR and REs
Gel Problems
Diagnosing Disorders w/ Gels
100
What is a DNA profile?
the unique genetic fingerprint of an individual
100
What does PCR do?
PCR splits the DNA into two single strands and then uses a special enzyme to create complementary strands of the single strands to rapidly multiply and magnify the amount of the DNA.
100
Who was at the crime scene based on the gel run on the DNA of samples found at the scene and from three persons of interest?
100
Who is a carrier? Diseased?
Erica is a carrier and Luis is has the disease.
200
What is the 'gel' in gel electrophoresis?
Agarose gel and a buffer solution
200
How do REs work?
Restriction enzymes cleave DNA at specific restriction sites. They cut the DNA at these spots and make smaller fragments of DNA.
200
Who is the mother based on the gel?
200
Explain the pedigree and gel. How does someone inherit the disease?
:)
300
How do you prepare a gel?
1. Prepare the agarose and buffer solution and place a comb on either sides of the gel
2. Cast into the tray
3. Let the gel solidify and then remove the combs
4. Place the tray on the gel box
5. Fill the gel box with the buffer so that a thin layer covers it.
300
What is a RFLP? How are they made?
RFLPs are the fragments created by REs and appear on gels.
300
What type of species is the sample's DNA from?
300
If a person must be homozygous to have cystic fibrosis then, based on the gel, which patient has abnormal CS genes?
google slides
400
How do you load a gel?
The first row on the negative end of the gel is the DNA ladder, with model RFLPs used to determine the length of the DNA samples' RFLPs. The next four or five rows are ones that will have the DNA samples from a disease, or a patient to study the structure of the DNA.
400
How are PCR, REs, and gel electrophoresis used to create DNA profiles?
With an unknown DNA strand, PCR is used to amplify the specific area that scientists want to study. Then, scientists can use REs to further isolate the specific sequence of nucleotides. These strands can then be run on a gel to determine their fragment lengths.
400
What is EcoR1's recognition site?
G/AATT
400
Based on the gel, which person has, doesn't have, or is a carrier for SCD?
google slides
500
How does gel electrophoresis work?
Because DNA is a negatively charged particle it is loaded in the negative end of the gel so that the fragments will move towards the positive end. The DNA fragments move based on their size; long fragments will move slowly through the pores of the agarose gel while short fragments will move quickly through the gel.
500
How are PCR and Restriction Enzymes related?
PCR is used to multiply the amount of a given DNA sample. After PCR, restriction enzymes can be used to further isolate the part of the DNA that is supposed to be studied or run for a gel.
500
What is HaeIII's recognition site?
GG/CC
500
Based on your previous answer, create the most likely pedigree.
google slides