Miscellaneous
What are the domains of life?
Bacteria, Archaea, Eukarya
What is the function of general purpose media?
To grow as many types of microbes as possible
What shape is a coccus?
sphere
What is the name of the central organelle of a eukaryotic cell where DNA is stored?
nucleus
Are viruses cellular?
No, they are acellular
What is a pathogen?
any microbe that causes disease
What is the name of the type of media which shows visual differences between microbes?
(Bonus points if you give an example)
Differential media
What structure can some bacteria form to withstand harsh conditions?
Endospore
Which two organelles divide independently of the cell?
Mitochondria and Chloroplasts
What does reverse transcriptase do?
What is the difference between sterile and pasteurized
sterile means completely free of living things, including viruses and prions.
pasteurized means most harmful germs were removed.
What are the 5 I's in order?
Inoculation, incubation, isolation, inspection, identification
Name the three parts of the bacterial flagellum
Which part of the eukaryotic cell anchors organelles and gives the cell shape?
The cytoskeleton
What are the two primary components of a virus?
genetic material
capsid - protein shell
What is positive chemotaxis?
a microbe moves toward a chemical signal
What does it mean for a microbe to be fastidious?
The microbe has very specific nutrient needs in order to grow.
Why do gram negative and gram positive cells stain different colors?
Gram positive cells have a thick layer of peptidoglycan in their walls that holds onto crystal violet.
Gram negative cells only have a thin layer, which does not keep the crystal violet as well.
What is the nucleolus?
darker center of the nucleus where ribosomal RNA is made.
Can one virus attack a wide variety of cell types?
No - viruses are very specific in which hosts they can attack - think of spikes and receptors
What is the equation for serial dilution, and what is an acceptable range for CFU?
Concentration = CFU/ (volume plated * dilution)
30-300
What is the difference between complex and defined media?
The exact chemical composition of defined media is known.
The exact chemical composition of complex media is not known
What is the term for multiple flagella arranged in a tuft on one end of a bacterium?
Lophotrichous
What are histones?
proteins that keep DNA compact in the nucleus
What is the difference between the lysogenic and the lytic cycle?
The bacteriophage kills the bacteria in the lytic cycle. In the lysogenic cycle, it incorporates its genome into the host's.